苣荬菜水煎液对糖尿病大鼠的降血糖作用及其机制

Hypoglycemic Effect and Mechanism of Endive Decoction on Diabetic Rats

目的观察苣荬菜水煎液对链脲佐菌素致糖尿病大鼠模型的降血糖作用及其机制。方法采用一次性腹腔注射链脲佐菌素的方法制作大鼠糖尿病模型,将大鼠分为空白组,模型组,二甲双胍组(0.47 g·kg^-1),苣荬菜水煎液高、中、低剂量组(3.34,1.67,0.83 g·kg^-1),实验中监测血糖,并检测各组大鼠血清中超氧化物歧化酶(superoxide dismutase,SOD)活力,丙二醛(malondialdehyde,MDA)、谷光甘肽(glutathione,GSH)、一氧化氮(nitric oxide,NO)、总胆固醇(total cholesterol,TC)、低密度脂蛋白(low density lipoprotein,LDL)、高密度脂蛋白(high density lipoprotein,HDL)、甘油三酯(triglyceride,TG)、糖基化血清蛋白(glycosylated serum protein,GSP)、胰岛素(insulin,INS)、胰岛素抗体(insulin antibody,IAA)水平和一氧化氮合酶(nitricoxidesynthase,NOS)活力,并采用HE染色法观察大鼠胰腺和肾脏组织病理变化。结果与空白组相比,模型组大鼠血糖值及血清NOS活力,MDA、NO、TC、LDL、TG、GSP、IAA水平显著升高(P<0.01),SOD活力,GSH、HDL、INS水平显著降低(P<0.01);与模型组相比,二甲双胍组和苣荬菜水煎液组各剂量均可不同程度降低血糖水平,二甲双胍组与苣荬菜水煎液高剂量组可显著升高SOD活力,GSH、HDL、INS水平(P<0.01),显著降低NOS活力,MDA、NO、TC、LDL、TG、GSP、IAA水平(P<0.01或P<0.05),改善胰腺、肾脏的病理变化。结论苣荬菜水煎液具有较好的降血糖效果,其作用机制与促进胰岛β细胞释放胰岛素有关,并可修复胰岛细胞组织、保护肾脏。

OBJECTIVE To observe the hypoglycemic effect and mechanism of endive decoction on streptozotocin induced diabetic rats.METHODS The diabetic rat model was established by intraperitoneal injection of streptozotocin.Rats were divided into normal group,control group,melbine group(0.47 g·kg^-1),endive decoction high,middle and low dose(3.34,1.67,0.83 g·kg^-1) group.The blood glucose was monitored and the activity of serum superoxide dismutase(SOD),the level of malondialdehyde(MDA),glutathione(GSH) and nitric oxide(NO),cholesterol(TC),low density lipoprotein(LDL),high density lipoprotein protein(HDL),triglyceride(TG),glycosylated serum protein(GSP),insulin(INS),insulin antibody(IAA) and the activity of nitric oxide synthase(NOS) was detected in each group.Pathological changes of pancreas and kidney in rats were observed by HE staining.RESULTS Compared with the blank group,the blood sugar,serum levels of MDA,NO,TC,LDL,TG,GSP,IAA and activity of NOS in the model group increased significantly(P<0.01),while the activity of SOD,level of GSH,HDL and INS decreased significantly(P<0.01).Compared with model group,melbine group and each dose of endive decoction groups could reduce blood sugar level in varying degrees.Melbine group and endive decoction high dose group could significantly increase SOD,GSH,HDL,INS level(P<0.01),significantly reduce NOS activity,MDA,NO,TC,LDL,TG,GSP,IAA level(P<0.01 or P<0.05),and improve pathological changes of pancreas and kidney.CONCLUSION Endive decoction has hypoglycemic effect.The mechanism of action is related to the promotion of insulin release from pancreatic β cells,and repair the islet cells,protect the kidney tissue.