丁苯酞联合亚低温治疗对兔心搏骤停后脑损伤的保护作用

Protective effects of butylphthalide combined with sub-hypothermia therapy on brain injury after cardiac arrest in rabbits

目的探讨丁苯酞联合亚低温治疗对兔心搏骤停后脑损伤的保护作用。方法将72只家兔分为对照组、亚低温组、丁苯酞联合亚低温组(联合组),每组24只。对照组心搏骤停复苏建模成功后无干预观察至72 h,亚低温组在心搏骤停复苏建模成功后给予亚低温治疗至72 h,联合组心搏骤停复苏术前168 h口服丁苯酞(40 mg·kg^-1·d^-1)到建模成功后72 h,同时给予亚低温治疗至72 h。3组家兔分别于心搏骤停复苏前及自主循环恢复(ROSC)后12、24、72 h进行神经功能缺损评分(NDS),然后每组各时间点处死3只家兔,检测血清神经元特异性烯醇化酶(NSE)、S-100B蛋白水平和脑组织含水量。结果亚低温组家兔复苏ROSC后12、24、72 h NDS均比对照组同时间点明显升高(P<0.05),NSE、S-100B均明显低于对照组同时间点水平(P<0.05);联合组家兔复苏ROSC后12、24、72 h NDS均比亚低温组同时间点明显升高(P<0.05),NSE、S-100B均明显低于亚低温组组同时间点水平(P<0.05);3组家兔只有复苏ROSC后72 h脑组织含水量比较,差异有统计学意义(P<0.05)。结论丁苯酞联合亚低温治疗对兔心搏骤停复苏后脑损伤具有保护作用。

Objective To investigate the protective effects of butylphthalide combined with sub-hypothermia therapy on brain injury after cardiac arrest in rabbits.Methods Seventy-two rabbits were randomly assigned to the control group,sub-hypothermia group and butylphthalide combined with sub-hypothermia group(combined group)with 24 rabbits in each group.After successfully constructing the model by cardiac arrest resuscitation,the control group was observed without intervention until 72 h,the rabbits in the sub-hypothermia group was given the sub-hypothermia therapy until 72 h,and the combined group orally took butylphthalide(40 mg·kg^-1·d^-1)at 168 h before cardiac arrest resuscitation until 72 h after successful model construction.The neurological deficit score(NDS)in 3 groups was performed before cardiac arrest resuscitation,and at 12,24,72 h after the restoration of spontaneous circulation(ROSC)and 3 rabbits were killed.The levels of serum neuron-specific enolase(NSE)and s-100B protein and the brain tissue water contents were measured.Results The NDS scores at 12,24,72 h after ROSC in the sub-hypothermia group were significantly increased compared with the control group at the same time points(P<0.05),while the NSE and S-100B levels in the sub-hypothermia group were significantly lower than those in the control group at the same time points(P<0.05).The NDS scores at 12,24,72 h after ROSC in the combined group were significantly increased compared with the sub-hypothermia group(P<0.05),while the NSE and S-100B levels were significantly lower than those in the sub-hypothermia group at the same time points(P<0.05).Only after ROSC resuscitation,the brain tissue water content of rabbits in the 3 groups was compared 72 h after ROSC resuscitation,and the difference was statistically significant(P<0.05).Conclusion Butylphthalide combined with sub-hypothermia treatment has the protective effect on the brain injury after cardiac arrest resuscitation in rabbits.