骨碎补对去卵巢大鼠骨微结构的保护作用

Protective effects of Rhizoma Drynariae on bone microarchitecture of ovariectomized rats

为观察骨碎补对去卵巢大鼠骨微结构的作用并探讨其可能作用机制,将36只大鼠分为实验组(OVXDF)、模型组(OVX)和假手术组(SHAM)。OVXDF及OVX组去卵巢造模,术后8周,测量3组大鼠骨密度后,OVXDF组给予骨碎补水煎液灌胃,OVX及SHAM组给予生理盐水灌胃,灌胃12周。然后利用Micro-CT测量骨密度BMD(g/cm^3)、骨矿含量BMC(g)、骨小梁骨量BV/TV(%)、骨小梁厚度Tb.Th(μm)、骨小梁数量Tb.N(mm^-1)、骨小梁分离度Tb.sp(mm)、结构模型指数SMI,显微镜下观察骨组织显微形态情况,酶联免疫吸附法测定大鼠血清TRAP、MMP-9、CTX-1含量。结果显示大鼠去卵巢后,不仅表现为骨量的降低,同时出现骨微细结构的变化,骨碎补灌胃后可以有效地对抗去卵巢大鼠的骨密度降低及骨微细结构的变化,其作用机制与其抑制骨髓脂肪细胞生成、抑制破骨细胞活性及数量有关。

To investigate the effects of Rhizome Drynariae on bone microarchitecture of ovariectomized rats and to explore its possible mechanisms. Thirty-six rats were divided into experimental group( OVXDF),model group( OVX),and sham operation group( SHAM). In the OVXDF and OVX groups,bilateral ovaries were removed. Eight weeks after operation,bone mineral density was measured. After this,the OVXDF group was administered Rhizoma Drynariae intragastrically,and the OVX and SHAM groups received normal saline intragastrically for 12 weeks. Subsequently,micro-CT was used to measure bone mineral density( g/cm^3),bone mineral content( g),trabecular bone volume over total volume( %),and trabecular thickness Tb.Th( μm),trabecular bone number Tb.N( mm^-1),trabecular bone separation degree Tb.sp( mm),and structural model index. Rat microscopic morphology of the bone tissue was observed under microscope.Concentrations of tartrate-resistant acid phosphatase,matrix metallopeptidase 9,and CTX-1 in rat serum were measured by enzyme-linked immunosorbent assay. The experiment results showed not only decreased bone mass but also changed bone microarchitecture in ovariectomized rats. Rhizoma Drynariae decocted with water intragastrically can effectively compensate for the decrease in bone mineral density and the change in bone microarchitecture in ovariectomized rats. Its mechanism of action is associated with its inhibition of bone marrow adipocyte formation and suppression of activity and number of osteoclasts.