黑土微生物群落对副球菌属（Paracoccus sp.)QD15-1修复邻苯二甲酸二甲酯污染土壤的响应

Response of microbial community in black soil to remediation of dimethyl phthalate contaminated soil by Paracoccus sp.QD15-1

采用Illumina MiSeq高通量测序法探究黑土微生物群落对副球菌属(Paracoccus sp.)QD15-1修复邻苯二甲酸二甲酯(DMP)污染土壤的响应。结果表明,副球菌属QD15-1在7d内可快速有效降解土壤中的DMP,平均降解率为94.7%,并能保持稳定;Shannon、Sobs指数显示,土壤中微生物多样性、丰富性受到DMP的影响;土壤中的优势门为放线菌门(Actinobacteria),占总菌门数的50%以上;鞘氨醇单胞菌属(Sphingomonas)是第一优势菌属,第二优势菌属为类诺卡氏菌属(Nocardioides)。

Illumina MiSeq high-throughput sequencing was used to study the response of microbial community in black soil to remediation of dimethyl phthalate(DMP)contaminated soil by Paracoccus sp.QD15-1.The results showed that Paracoccus sp.QD15-1 could degrade DMP in soil quickly and effectively within 7 days.It had an average degradation rate of 94.7%,and remained stable for Shannon index and Sobs index showed that the microbial diversity and richness of soil both affected by DMP.The dominant phylum in the soil was Actinobacteria,which accounted more than 50% of the total phyla.Sphingomonas was the first dominant genus,and Nocardioides was the second one.