摘要
目的建立了一种快速检测尿样中61种毒品及滥用药物的超高效液相色谱串联质谱(UPLC-MS/MS)方法。方法尿样按19(体积比)经甲醇沉淀蛋白、离心处理,采用电喷雾离子源正离子(ESI+)模式和多反应检测(MRM)模式进行质谱分析。结果61种化合物在0.1~10ng/mL浓度范围内均获得良好的线性,相关系数均在0.99以上,该方法的提取回收率分布在90.14%~129.72%之间,最小检测限可达0.05ng/mL。结论本方法灵敏、简便、快速,适用于尿样中毒品及滥用药物的定性定量分析。
Objective A rapid and sensitive method of ultra-performance liquid chromatography coupled with mass spectrometry(UPLC-MS/MS) was to develop for simultaneous determination of 61 abused drugs in urine. Methods Urine sample was precipitated with methanol under the ratio of 1:9(V:V), afterwards centrifuged at 8500 r/min for 10 min, with the supernatant being analyzed through UPLC-MS/MS in coalescence of an electrospray ionization(ESI) source. The samples were measured in the mode of electrospray positive ionization(ESI+) and multiple reaction monitoring(MRM). Results The 61 drugs were linear in the range of 0.1~10 ng/m L, with their coefficients higher than 0.99. The extraction recoveries ranged from 90.14%~129.72% for all of the analytes, and their limits of detection(LODs) were 0.05 ng/m L. Conclusions The method presented here is validated to be simple, rapid, sensitive and accurate for determination of trace drugs and metabolites in urine samples.
作者
刘帅
赵蒙
杨发震
李绍鹏
王朝虹
李虹
LIU Shuai;ZHAO Meng;YANG Fazhen;LI Shaopeng;WANG Zhaohong;LI Hong(Institute of Forensic Medicine,Kunming Medical University,Kunming 650500,China;Center of Procuratoral Technology and Information,Supreme People’s Procuratorate,Beijing 100144,China;Yuxi Public Security Bureau,Yuxi 653100,Yunnan,China;Key Laboratory of Narcotics Assay and Control Technology,Ministry of Public Security,Kunming 650228,China)
出处
《刑事技术》
2020年第1期45-50,共6页
Forensic Science and Technology
基金
公安部科技强警基础工作专项项目（2016GABJC50）
