超声引导清宫术下的早期胚胎组织的亲权鉴定

Paternity Identification of Early Embryo Tissues Collected under Ultrasound-guided Complete Curettage of Uterine Cavity

经超声引导清宫术获得的人工流产物大多为全部胚胎组织与母体组织的混合物。只有从这些混合物中辨认、分离出胚胎组织才能成功进行亲权鉴定。但由于孕早期胚胎过小、分化程度不明显,且经该手术的人工流产组织往往破碎,从而使分离胚胎组织进行取样的难度较大。本文通过1例超声引导清宫50d左右的人工流产胚胎组织的亲权鉴定,分析总结处理该案的要点,认为在这类案件中为能够更准确地选取人工流产物中的胚胎组织,应以蒸馏水细水反复清洗流产物至水清;取材应优先考虑提取绒毛膜组织进行DNA检测;多样化取材,如此可提高检出成功率。

Ultrasound-guided complete curettage of uterine cavity is often performed with gynaecology and obstetrics in clinical diagnosis and treatment. Most of the abortion materials obtained by such an operation are a mixture of all embryonic tissues and the mother’s. Therefore, a successful paternity identification depends on the embryonic tissues to be successfully recognized and isolated from the mixture. However, due to the small size of the early embryos, their low differentiation and the fragmentation of the abortion tissues undergone through operation, it is difficult to separate the embryonic tissues for sampling. This paper reports a case about paternity identification for a 50-day embryo collected with ultrasound-guided complete curettage of uterine cavity. According to the analysis of the main points summarized from this case, the following measures are considered to be condignly taken of, 1. gently washing the abortion materials repeatedly with distilled water until the arrival of clear eluate;2. preferentially extracting villus/villi from chorionic tissues for embryonic DNA detection;3. sampling at various sites and of diversity, thus the probability of success can be improved.