摘要
目的探讨大鼠骨髓间充质干细胞(BMSC)分泌的外泌体(BMSC-Exo)对原代软骨细胞迁移、增殖和凋亡的影响。方法超速离心结合超滤浓缩法提取BMSC-Exo,通过透射电镜和Western blot法鉴定外泌体。分别使用浓度为25、50和100μg/ml的BMSC-Exo处理软骨细胞,划痕实验检测软骨细胞的迁移,CCK-8法检测软骨细胞的增殖,流式细胞术检测软骨细胞的凋亡。为模拟骨关节炎(OA)病理状态,同时设置IL-1β(10ng/ml)对照组。结果与细胞培养基对照组比较,BMSC-Exo作用使软骨细胞的划痕愈合率显著升高(12h,P<0.05;24h,P<0.01),细胞增殖速度明显加快(P<0.01),细胞凋亡比例显著降低(P<0.01),且均存在时间和剂量依赖性。IL-1β刺激可抑制软骨细胞的迁移和增殖,促进软骨细胞的凋亡,而BMSC-Exo可显著削减IL-1β的作用效果。结论BMSC-Exo可促进软骨细胞的迁移运动和增殖能力,抑制软骨细胞发生凋亡,可对软骨细胞的生长发挥重要的调控作用。
Objective To investigate the effects of rat bone marrow mesenchymal stem cell-derived exosome(BMSC-Exo)on the migration,proliferation and apoptosis of chondrocytes.Methods BMSC-Exo were isolated and identified by transmission electron microscope and Western blot.Primary chondrocytes were treated with different concentrations of BMSC-Exo(25,50 and 100μg/ml),and the cell scratch assay,CCK-8 assay and flow cytomertry method were used to assess the migration,proliferation and apoptosis of chondrocytes respectively.To simulate the pathological status of osteoarthritis(OA),the IL-1βcontrol groups were set at the same time.Results Compared with control groups,the scratch healing and cell proliferation ability were significantly increased,the apoptotic rates were obviously reduced of cells in BMSC-Exo treated groups.IL-1βstimulation could inhibit the migration and proliferation of chondrocytes,promote the apoptosis of chondrocytes,while BMSC-Exo could significantly reduce the effects of IL-1β.Conclusion BMSC-Exo could promote the migration and proliferation of chondrocytes,and inhibit the apoptosis of chondrocytes,such play an important role in the growth regulation of chondrocytes.
作者
孟迪
景金珠
王莹
梁震
Meng Di;Jing Jinzhu;Wang Ying(Beijing Institute of Traumatology and Orthopaedics,Beijing Jishuitan Hospital,Beijing 100035,China)
出处
《医学研究杂志》
2020年第1期61-66,共6页
Journal of Medical Research
基金
国家自然科学基金资助项目(81601947)。