吲哚美辛对骨肉瘤143B细胞增殖、迁移和凋亡的影响

Effects of Indomethacin on Proliferation, Migration and Apoptosis of Osteosarcoma 143B Cells

目的观察吲哚美辛对骨肉瘤143B细胞增殖、凋亡的影响,并探讨其可能的机制。方法体外培养人骨肉瘤细胞143B,倒置显微镜观察骨肉瘤143B细胞的形态;CCK-8检测骨肉瘤143B细胞增殖情况;流式细胞术分析骨肉瘤143B细胞的凋亡;Transwell检测骨肉瘤143B细胞迁移情况;Western blot法检测细胞凋亡相关蛋白Bcl-2的表达。结果CCK-8结果显示吲哚美辛能抑制骨肉瘤143B细胞的增殖,呈时间、浓度依耐性;流式细胞术结果显示吲哚美辛以浓度依赖的形式诱导骨肉瘤细胞凋亡;Transwell结果显示吲哚美辛可以抑制骨肉瘤143B细胞迁移;Western blot法检测结果表明,吲哚美辛下调抑凋亡蛋白Bcl-2的表达。结论吲哚美辛抑制骨肉瘤143B细胞增殖和迁移能力并且促进其凋亡,并呈现时间和浓度依赖性。

Objective To observe the effect of indomethacin on proliferation and apoptosis of osteosarcoma 143B cells and explore the possible mechanism.Methods Human osteosarcoma 143B cells were cultured in vitro.The morphology of human osteosarcoma 143B cells was observed by inverted microscope.CCK-8 was used to detect the proliferation of osteosarcoma 143B cells.Flow cytometry was used to analyze the apoptosis of osteosarcoma 143B cells.Transwell assay showed osteosarcoma 143B cell invasion.Expression of Bcl-2 was detected by Western blot.Results CCK-8 results showed that indomethacin could inhibit the proliferation of osteosarcoma 143B cells in a time-dependent and concentration-dependent manner.Flow cytometry showed that indomethacin induced apoptosis of osteosarcoma cells in a concentration-dependent manner.Transwell results showed that indomethacin inhibited the migration of osteosarcoma 143B cells.Western blot analysis showed that indomethacin down-regulated the expression of anti-apoptotic protein Bcl-2.Conclusion Indomethacin inhibits proliferation and migration and promotes apoptosis of osteosarcoma 143B cells in a time-dependent and concentration-dependent manner.