VEGF、MMP-9、Galectin-3在卵巢子宫内膜异位症发病中的作用及临床应用价值

The Role and Clinical Value of VEGF, MMP-9 and Galectin-3 in the Pathogenesis of Ovarian Endometriosis

目的:探讨血清血管内皮生长因子(vascular endothelial growth factor,VEGF)、半乳糖凝集素-3(Galectin-3)、基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)在卵巢子宫内膜异位症(endometriosis,EMs)中的表达及临床应用价值。方法:选取卵巢子宫内膜异位症患者32例为EMs组,妇科良性疾病且排除EMs患者40例为对照组。采用酶联免疫吸附试验技术检测EMs组与对照组患者术前血清中VEGF、Galectin-3、MMP-9的表达情况,采用ROC曲线分析三因子在卵巢EMs诊断中的临床应用价值。结果:EMs组血清中VEGF、Galectin-3及MMP-9的表达水平均高于对照组(P<0.05)。EMs组血清中VEGF、Galectin-3及MMP-9在Ⅲ、Ⅳ期患者中的表达水平均高于Ⅰ、Ⅱ期患者(P<0.05)。ROC曲线分析显示,VEGF、Galectin-3、MMP-9在EMs中诊断的灵敏度分别为75.0%、84.4%、75.0%,特异度分别为70.0%、52.5%、77.5%。结论:血清VEGF、Galectin-3、MMP-9可能与EMs的发生、发展有关,检测血清中VEGF、Galectin-3、MMP-9的表达水平对卵巢EMs早期诊断、病情评估及随访有临床应用价值。

Objective:To investigate the value of serum vascular endothelial growth factor(VEGF),Galectin-3,matrix metalloproteinase-9(MMP-9)in the diagnosis for patients with ovarian endometriosis(EMs).Method:32 patients diagnosed as ovarian endometriosis were defined as EMs group,40 patients diagnosed as gynecological benign disease and excluded EMs were defined as control group.The levels of serum VEGF,Galectin-3,MMP-9 were determined by enzyme-linked immunosorbent assay(ELISA)for EMs group and control group.The receiver operating characteristics(ROC)curve analysis was performed to analyze the value of VEGF,Galectin-3 and MMP-9 in the diagnosis of EMs.Result:The levels of serum VEGF,MMP-9 and Galectin-3 in EMs group were signincantly higher than those in control group(P<0.05).The levels of serum VEGF,MMP-9 and Galectin-3 in patients with stage Ⅲ and Ⅳ in EMs group were significantly higher than those in patients with stage Ⅰ,Ⅱ(P<0.05).The results of ROC curve analysis showed that the diagnostic sensitivity and specificity of VEGF,Galectin-3,MMP-9 were 75.0%,84.4%,75.0% and 70.0%,52.5%,77.5%,respectively.Conclusion:VEGF,Galectin-3 and MMP-9 likely to be the pathogenesis of EMs,the detection of serum VEGF,Galectin-3 and MMP-9 can be used as indicators for early diagnosis,assessment and follow-up of EMs.