板栗疫病菌cpfluG基因的功能研究

Functional Study of cpfluG Gene in Chestnut Blight Fungus(Cryphonectria parasitica)

fluG基因在许多丝状真菌中对无性孢子的发育起调控作用,然而在树木病原菌板栗疫病菌(Cryphonectria parasitica)中,无性孢子的发育调控机制尚未完全明确。本研究克隆了板栗疫病菌的fluG同源基因cpfluG,采用同源重组的方法构建了fluG基因缺失菌株ΔcpfluG。相对于野生型菌株,ΔcpfluG突变株几乎完全丧失产孢能力,色素分泌显著减少,但致病性不受影响。cpfluG基因的缺失导致已知的产孢相关基因cls-31表达量显著下调,但不影响pks基因和cls-32的表达。本研究结果揭示,fluG是板栗疫病菌孢子发育调控的一个关键成员,但板栗疫病菌分生孢子发育的调控细节可能有别于已报道的丝状真菌。

The fluG gene plays a regulatory role in the development of asexual spores in many filamentous fungi.However,in the tree pathogen Cryphonectria parasitica,the developmental regulation mechanism of asexual spores has not been fully clarified.In this study,the fluG homologous gene cpfluG in Cryp honectria parasitica was cloned,and the fluG gene deletion strainΔcpfluG was constructed by homologous recombination.Compared with the wild-type strain,theΔcpfluG mutant strain almost completely lost the ability to sporulate,and the pigment secretion was significantly reduced,but the pathogenicity was not affected.Deletion of the cpfluG gene resulted in a significant down-regulation of the known sporulation-related gene cls-31,but did not affect the expression of the pks gene and cls-32 gene.The results of this study revealed that fluG is a key member of spore development regulation in Cryphonectria parasitic a,but the regulation of conidial development in Cryphonectria parasitica may be different from the reported filamentous fungi.