阿胶粗多糖脱蛋白方法比较及抑制酪氨酸酶活性研究

Comparison of Deproteinization Methods of Crude Polysaccharide of Donkey-hide-gelatin and Inhibition on Tyrosinase Activity

[目的]研究阿胶多糖的分离提纯工艺及其抑制酪氨酸酶活性。[方法]针对阿胶多糖提取中蛋白质的干扰,通过对几种脱蛋白方法(TCA法、Sevage法、蛋白酶法)的比较,优选出阿胶多糖提取液的脱蛋白方法,并探讨阿胶多糖对酪氨酸酶的抑制作用。[结果]TCA法、Sevage法及蛋白酶法的脱蛋白率分别为78.94%、87.73%和29.97%,多糖保留率分别为73.96%、32.14%和72.57%。所得阿胶多糖对酪氨酸酶有一定的抑制作用,当浓度为1 mg/mL时,抑制率为24.42%。[结论]TCA法脱蛋白率较高、多糖损失率较低,筛选方法可有效去除阿胶粗多糖中的蛋白,所得阿胶多糖具有良好的抑制酪氨酸酶作用。

[Objective]The research aimed to study the separation and purification process of crude polysaccharide of donkey-hide-gelatin and its inhibition of tyrosinase activity.[Method]Aiming at the interference of protein in the extraction of donkey-hide-gelatin polysaccharides,the deproteinization method of donkey-hide-gelatin polysaccharide extract was optimized by comparing several deproteinization methods(TCA method,Sevage method and protease method),the deproteinization method of donkey-hide-gelatin polysaccharide extract was optimized,and the inhibitory effect of donkey-hide-gelatin polysaccharide on tyrosinase was discussed.[Result]The deproteinization rates of TCA,Sevage and protease were 78.94%,87.73%and 29.97%,respectively,and the polysaccharide retention rates were 73.96%,32.14%and 72.57%,respectively.The obtained gelatin polysaccharide had a certain inhibitory effect on tyrosinase.When the concentration was 1 mg/mL,the inhibition rate was 24.42%.[Conclusion]The TCA method has higher deproteinization rate and lower polysaccharide loss rate.The screening method can effectively remove the protein from the crude polysaccharide of donkey-hide-gelatin,and the obtained donkey-hide-gelatin polysaccharide has a good inhibitory effect on tyrosinase.