利用废革屑制备不同分子量肽-Zn螯合物的工艺探究

Study on the Technology of Preparing Polypeptide Zinc Chelate from Leather Waste

本文主要通过不同用量的碱对废革屑进行水解,制备不同分子量的多肽1、多肽2、多肽3,利用GPC测定3种多肽分子量,并将多肽和Zn(CH3COO)2进行螯合反应来制备多肽-Zn金属螯合物。设计单因素实验,考察了pH、反应温度、反应时间、多肽-Zn质量比等因素对螯合反应的影响。确定了Zn(CH3COO)2与多肽螯合的最佳条件。多肽1的最佳螯合条件:多肽-Zn比为7∶1,反应时间60 min,反应温度为60℃,pH为8,最佳条件下的螯合率为66.83%;多肽2的最佳螯合条件:多肽-Zn比为7∶1,反应时间60 min,反应温度为60℃,pH为8,最佳条件下的螯合率为75.33%;多肽3的最佳螯合条件:多肽-Zn比为6∶1,反应时间60 min,反应温度为60℃,pH为7,最佳条件下的螯合率为82.56%;最后,对多肽-Zn螯合物进行红外光谱分析表征,结果表明Zn离子成功螯合到多肽上,得到Zn肽螯合物。

Different molecular weights polypeptide 1,polypeptide 2 and polypeptide 3 were synthesized by using different amounts of sodium hydroxide hydrolyzing leather waste in this paper,and the molecular weights of three polypeptides were determined by GPC.Polypeptide-Zn metal chelate were prepared by the chelation reaction of polypeptide and Zn(CH3COO)2.The influence factors on the chelation reaction such as pH,temperature,time,polypeptide zinc mass ratio and other factors were explored through the single factor experiment,and the optimal conditions of reaction were identified.Optimal chelating conditions for polypeptide 1 were polypeptide zinc mass ratio of 7∶1,60 min,60℃,pH 8,and the chelating rate under optimal conditions was 66.83%.Optimal chelating conditions for polypeptide 2 were polypeptide zinc mass ratio of 7∶1,60 min,60℃,pH 8,and the chelating rate under optimal conditions was 75.33%.Optimal chelating conditions for polypeptide 3 were polypeptide zinc mass ratio of 6∶1,60 min,60℃,pH 7,and the chelating rate under optimal conditions was 82.56%.Finally,the polypeptide zinc chelate was characterized by Fourier Transform Infrared(FTIR).The results showed that zinc ions were successfully chelated to the polypeptide to obtain polypeptide zinc chelate.