安康鱼肠中胶原蛋白与多糖的提取与保湿性

Extraction and Hygroscopic Properties of Collagen Protein and Polysaccharide in Lophius Litulon Intestines

胶原蛋白经改性可作为皮革的复鞣剂开发利用,具有绿色环保、安全无毒害等特点。用酶法从废弃的安康鱼鱼肠中提取了胶原蛋白和多糖,研究了样品的预处理及胶原蛋白和多糖提取的最佳条件。胶原蛋白的最佳提取条件是:pH 1.9、料液比1∶10、胃蛋白酶的添加量为1%、酶解时间4 h、酶解温度10℃,其提取率为11.3%;多糖的最佳提取条件是:料水比1∶5、温度90℃、提取时间2 h,其提取率为1%。用提取的胶原蛋白和多糖涂抹皮肤,测试了保湿性,胶原蛋白和多糖的含水量分别为38.40%和37.20%。

Collagen can be modified and used as a retanning agent for leather.It is green,safe and non-toxic.Collagen and polysaccharide were extracted fromthe intestines of discarded Lophius litulon by enzymatic method.The pretreatment of samples and the optimal conditions for collagen and polysaccharide extraction were studied.The optimal extraction conditions for collagen were:pH 1.9,ratio of material to liquid 1∶10,addition amount of pepsin 1%,enzymatic hydrolysis time of 4 h,enzymatic hydrolysis temperature of 10℃,and extraction rate of 11.3%;the extraction conditions for polysaccharide were as follows:the ratio of material to water was 1∶5,the temperature was 90℃,the extraction time was 2 h,and the extraction rate was 1%.The skin was moistened with the extracted collagen and polysaccharide,and the water content of collagen and polysaccharide was 38.40%and 37.20%.