长尾仓鼠VKORC1基因的提取与扩增

Extraction and Amplification of VKORC1 Gene in Cricetulus longicaudatus

为了探究长尾仓鼠VKORC1基因与其抗药性的关系,以长尾仓鼠的基因组DNA为模板,使用特异引物对长尾仓鼠的VKORC1基因片段进行扩增并获得其序列。结果表明,使用2对引物可分别扩增出长度为700、600 bp的条带;条带与预测基因片段大小一致,为目标条带。研究应用分子生物学手段探索抗药性监测的新途径,可为进一步获得该基因的全长,并探究VKORC1基因与抗药性的关系奠定基础。

To explore the relationship between VKORC1 gene and resistance of Cricetulus longicaudatus, the paper amplified the VKORC1 gene fragment of Cricetulus longicaudatus and obtained its sequence. The VKORC1 gene fragment was amplified with specific primers using the genomic DNA of Cricetulus longicaudatus as template. The result showed that two pairs of primers were used to amplify700, 600 bp bands, respectively. The size of the bands was the same as that of the predicted gene segments, which was the target band. In this study, molecular biological methods were used to explore a new way of resistance monitoring, which laid a foundation for further obtaining the full length of the gene and researching the relationship between VKORC1 gene and resistance.