GAP1基因缺失对上面发酵酵母高级醇代谢能力的影响

Effect of GAP1 Gene Deletion on Higher Alcohols Production in Top-fermenting Yeast

以亲本上面发酵酵母工业菌株S17为对照,研究了GAP1一个等位基因敲除重组菌株S17G和GAP1两个等位基因敲除重组菌株S17G2的发酵性能.实验结果表明:与亲本菌株相比,重组菌株S17G的总高级醇生成量降低17.47%,α氨基氮利用能力降低13.67%,麦芽糖利用能力降低31.08%;重组菌株S17G2的总高级醇生成量得到进一步降低,达到210.58 mg/L,较亲本菌株降低了21.98%,α氨基氮利用能力降低了18.47%,麦芽糖利用能力降低了35.14%.重组菌株S17G、S17G2的其他发酵性能与亲本菌株S17相比没有发生显著的变化.

The fermentation performances of the mutant strain S17G with one GAP1 allele deletion and the mutant strain S17G2 with two GAP1 allele deletion were investigated.The results showed that the mutants have the following advantages over the parental strain S17.The production of higher alcohols by S17G was 17.47%lower than that of the parental strain S17.The utilizations ofα-amino nitrogen and maltose in the mutant strain S17G decreased by 13.67%and 31.08%,respectively,compared with the parental strain.The concentration of higher alcohols decreased to 210.58 mg/L in the mutant S17G2,reduced by 21.98%compared with the strain S17;α-amino nitrogen and maltose utilization in the mutant strain S17G2 were 18.47%and 35.14%lower than that of S17,respectively.Other fermentation performances of the mutants S17G and S17G2 were consistent with the parental strain S17.