不同浓度氯化钴对大鼠脑微血管内皮细胞的损伤作用

Damage effects of cobalt chloride in different concentrations on microvascular endothelial cells of rat brain

目的研究不同浓度的氯化钴(CoCl2)对大鼠脑微血管内皮细胞(BMECs)细胞的损伤作用。方法利用不同浓度的(0、100、200、400、800μmol/L)CoCl2建立大鼠脑微血管内皮细胞缺氧模型。通过CCK-8法检测CoCl2对大鼠脑微血管内皮细胞的体外生长增殖活性的影响。TUNEL染色检测细胞凋亡;采用化学比色法测定细胞内丙二醛(MDA)、谷胱甘肽(GSH)含量及超氧化物歧化酶(SOD)的活性。结果 CCK-8显示CoCl2对BMECs细胞增殖抑制作用表现出浓度依赖性抑制作用。TUNEL染色结果显示,随着CoCl2剂量增加,氯化钴处理组的的细胞凋亡比较对照组显著升高(P <0.05)。化学比色检测结果显示,CoCl2浓度的升高可显著增加细胞内MDA含量,减少GSH含量及SOD活性(P <0.01)。结论 CoCl2可抑制细胞的增殖,同时CoCl2可呈浓度依赖性损伤大鼠脑微血管内皮细胞,其可能与CoCl2增加诱导细胞凋亡、引起氧化应激反应等损伤有关。

Objective To investigate the effect of different concentrations of cobalt chloride(CoCl2)on the damage of rat brain microvascular endothelial cells(BMECs)cells. Methods Hypoxic cell models were established by CoCl2 with different concentration(0、100、200、400、800 μmol/L). CCK-8 assay was used to detect the proliferative of BMECs. TUNEL staining was used to detect apoptosis;Chemical colorimetric was used to detect the level of malondialdehyde(MDA), glutathione(GSH)content and superoxide dismutase(SOD)activity. Results CCK-8 showed that CoCl2 inhibited the proliferation of BMECs cells in a concentration-dependent manner. TUNEL staining showed that with the increase of CoCl2 dose, apoptosis was significantly higher in the cobaltous chloride treatment group than in the control group(P < 0.05). The results of chemical colorimetry showed that the increase of CoCl2 concentration significantly increased the content of MDA and decreased the content of GSH and SOD(P = 0.01). Conclusion CoCl2 can inhibit cell proliferation,and CoCl2 can damage brain microvascular endothelial cells in rats in a concentration-dependent manner, which may be related to increased apoptosis induced by CoCl2 and oxidative stress response.