长链非编码RNA母系表达基因3通过调控PTEN抑制膀胱尿路上皮癌细胞增殖活性的分子研究

LncRNA MEG3 suppresses cell proliferation activity of bladder urothelial carcinoma through mediating PTEN

目的探究膀胱尿路上皮癌中长链非编码RNA(lncRNA)母系表达基因3(MEG3)的表达情况以及MEG3调控膀胱尿路上皮癌细胞增殖活性的分子机制。方法通过实时荧光定量PCR检测膀胱尿路上皮癌组织及癌旁正常组织中lncRNA MEG3的表达水平;通过Western blot检测过表达lncRNA MEG3后T24细胞内第10号染色体同源丢失性磷酸酶一张力蛋白基因(PTEN)蛋白水平;CCK8试剂盒检测细胞活性。结果MEG3的RNA水平在膀胱尿路上皮癌组织中表达显著下调(P<0.01),PTEN的表达水平也明显降低(P<0.01)。T24中过表达MEG3后,PTEN水平上调,细胞活性降低(P<0.01);而过表达MEG3并给予PTEN抑制剂时细胞活性则上升。结论过表达lncRNA MEG3可通过上调PTEN抑制人膀胱尿路上皮癌T24细胞的细胞增殖活性,有可能成为临床治疗的潜在靶点。

Objective To study the expression of long non-coding RNA(lncRNA)maternally expressed 3(MEG3)in urothelial carcinoma and the molecular mechanism of MEG3 mediating cell proliferation activity.Methods Real-time fluorescence quantitative PCR(RT-PCR)was used to detect the expressions of MEG3 in urothelial carcinoma and normal adjacent tissues.The level of and gene of phosphate and tension homology deleted on chromosome ten(PTEN)in T24 cells after overexpressing lncRNA MEG3 was detected by Western blot.And the cell proliferation activity was determined by CCK8 kit.Results The expression of MEG3 was significantly down-regulated in urothelial carcinoma tissues(P<0.01),and the expression of PTEN was also significantly down-regulated(P<0.01).After overexpression of MEG3 in T24,the level of PTEN increased and the cell activity decreased(P<0.01).And when PTEN inhibitor was given the same time,the cell activity increased.Conclusions Overexpression of lncRNA MEG3 can inhibit the proliferation of human urothelial carcinoma cell line T24 by up-regulating PTEN,which may become a potential target for clinical treatment.