贵州壮侗语族7个少数民族21个Y-SNP多态性

Single nucleotide polymorphism of 21 Y-chromosomal loci in seven ethnic minorities of Zhuang-Dong Language in the Guizhou Province of China

调查贵州壮侗语族7个少数民族(水族、布依族、侗族、仡佬族、壮族、毛南族、仫佬族)人群Y染色体上21个单核苷酸多态性(single nucleotide polymorphism,SNP)位点的遗传多态性,并探讨该人群与其他民族以及其他语族之间的遗传关系。采用SNapShot法对445例贵州壮侗语族7个少数民族男性个体21个Y-SNP位点进行复合扩增检测,用直接计数法计算等位基因频率、单倍型频率与单倍群频率,运用SPSS 24软件进行主成分分析。在贵州水族、布依族、侗族、仡佬族、壮族、毛南族和仫佬族中,21个Y-SNP组成的单倍型多态性值(haplotype diversity,HD)分别为0.5977、0.9456、0.8856、0.8869、0.7686、0.3587和0.7078;与已报道的其他9个民族人群单倍群分布频率进行主成分分析,壮侗语族与苗瑶语族聚在一起。从父系遗传角度分析,壮侗语族与苗瑶语族亲缘关系较近。

The study aimed to determine single nucleotide polymorphism of 21Y-chromosomal loci in seven ethnic minorities,including Shui,Bouyei,Dong,Gelo,Zhuang,Maonan and Mulam,of Zhuang-Dong language in the Guizhou province of China,and explore differences in the polymorphism from other Language ethnics.The SNapShot method was used to detect the complex amplicons of the 21 Y-SNPs loci in 445 males from seven Guizhou Zhuang-Dong language ethnics.Frequencies of alleles,haplotypes,and haploid populations were calculated using a direct counting method.SPSS 24 was used for principal component analyses.The haplotype diversity values were 0.5977,0.9456,0.8856,0.8869,0.7686,0.3587 and 0.7078,respectively,in the seven ethnics.We performed principal component analyses based on the frequencies of haplogroups by comparing our data with those reported for other nine ethnic groups in China.The results showed that Zhuang-Dong Language and Hmong-Mien Language were clustered together.From the perspective of patrilineal genetics,the relationship of Zhuang-Dong Language to Hmong-Mien Language is relatively closer than those of other ethnics.