长链非编码RNA HOXA11-AS在结直肠癌患者血清中的表达和意义

Expression and significance of long-chain non-coding RNA HOXA11-AS in serum of patients with colorectal cancer

目的利用实时荧光定量PCR建立一种检测血清HOXA11-AS的方法,并探索其在诊断、治疗结直肠癌中的效果及预后价值。方法应用实时荧光定量PCR法检测53例结直肠癌初诊患者、16例结直肠癌手术患者、42例结直肠息肉患者、20例结直肠癌术后复发或转移患者和40例体检健康者血清HOXA11-AS水平。结果结直肠癌初诊患者HOXA11-AS水平M(P25~P75)为2.69(1.69~3.49),显著高于结直肠息肉患者(t=6.622,P<0.05)和体检健康者(t=6.535,P<0.05);结直肠癌术后复发或转移患者HOXA11-AS水平显著高于结直肠癌初诊患者(t=7.141,P<0.05)和结直肠癌手术患者(t=4.673,P<0.05);追踪16例患者血清HOXA11-AS表达各异,但总体术后较术前呈下降的趋势(t=3.666,P=0.000);HOXA11-AS高表达组患者的总体生存率明显低于HOXA11-AS低表达组患者(t=2.463,P<0.05);结直肠癌初诊患者受试者工作特征曲线下面积为0.8672,将1.495设为临界值时,HOXA11-AS作为结直肠癌血清诊断标志物的灵敏度为80.00%,特异度为83.02%,阳性预测值为82.49%,阴性预测值为85.59%。结论实时荧光定量PCR法检测血清HOXA11-AS稳定可靠,HOXA11-AS可作为新型分子诊断标志物,辅助诊断结直肠癌,评价治疗效果和评估预后。

Objective To establish a method for detecting serum HOXA11-AS by real-time fluorescent quantitative PCR and explore its effect evaluation and prognosis in the diagnosis and treatment of colorectal cancer.Methods The serum HOXA11-AS levels of 53 newly diagnosed patients with colorectal cancer,16 patients with colorectal cancer surgery,42 patients with colorectal polyps,20 patients with recurrence or metastasis of colorectal cancer and 40 healthy people were detected by real-time fluorescence quantitative PCR.Results The HOXA11-AS level of M(P25-P75)of newly diagnosed colorectal cancer patients was 2.69(1.69-3.49),which was significantly higher than that of patients with colorectal polyps(t=6.622,P<0.05)and those who were healthy in physical examination(t=6.535,P<0.05).The levels of HOXA11-AS in patients with recurrence or metastasis of colorectal cancer were significantly higher than those in newly diagnosed colorectal cancer(t=7.141,P<0.05)and patients with colorectal cancer surgery(t=4.673,P<0.05).The expressions of HOXA11-AS in serum of 16 patients were different,but the overall postoperative showed a downward trend compared with preoperative(t=3.666,P=0.000).The overall survival rate of patients with HOXA11-AS high expression group was significantly lower than that of patients with HOXA11-AS low expression group(t=2.463,P<0.05).When the area under receiver operating characteristic curve of newly diagnosed patients with colorectal cancer was 0.867 2,and 1.495 was set as the critical value,the sensitivity,specificity,positive predictive value and negative predictive value of HOXA11-AS were 80.00%,83.02%,82.49% and 85.59%,respectively.Conclusion Serum HOXA11-AS can be stably and reliably detected by real-time fluorescent quantitative PCR,and can be used as a new molecular diagnostic marker to assist in the diagnosis of colorectal cancer,evaluate the treatment effect and evaluate the prognosis.