他莫昔芬对胃癌HGC-27细胞Mcl-1siRNA、miR-302a表达的影响

Effects of tamoxifen on the expression of Mcl-1 siRNA and miR-302a in gastric cancer HGC-27 cells

目的分析他莫昔芬对胃癌HGC-27细胞髓样细胞白血病-1蛋白(Mcl-1)siRNA、micorRNA-302a(miR-302a)表达的影响。方法将细胞分为对照组、他莫昔芬0、50、100、200、400μg/mL组,检测细胞增殖及凋亡情况;检测细胞中Mcl-1siRNA、miR-302a水平;检测血管内皮生长因子(VEGF)及纤维细胞生长因子b(bFGF)水平。结果随着他莫昔芬给药量增加,HGC-27细胞增殖活性显著降低(P<0.05),早期凋亡率、晚期凋亡率及总凋亡率均明显升高,差异有统计学意义(P<0.05);HGC-27细胞Mcl-1siRNA水平均显著降低(P<0.05),miR-302a水平均显著升高(P<0.05);HGC-27早期VEGF及bFGF水平均明显降低,差异有统计学意义(P<0.05)。结论采用他莫昔芬对胃癌HGC-27细胞干预后可有效提高细胞中miR-302a表达并降低细胞中Mcl-1siRNA水平。

Objective To analyze the effect of tamoxifen on the expression of myeloid cell leukemia-1(Mcl-1)siRNA and microR-302 a(miR-302 a)in gastric cancer HGC-27 cells.Methods The cells were divided into control group,tamoxifen 0,50,100,200 and 400μg/mL groups,then the proliferation and apoptosis of the cells were detected,the levels of Mcl-1 siRNA and miRNA-302 ain the cells were detected,and the levels of vascular endothelial growth factor(VEGF)and b fibroblast growth factor(bFGF)in the medium were detected.Results With the increase of tamoxifen dosage,the proliferative activity of HGC-27 cells decreased significantly(P<0.05),the early apoptosis rate,late apoptosis rate and total apoptosis rate increased significantly(P<0.05);the Mcl-1 siRNA level of HGC 27 cell decreased significantly(P<0.05),and the level of miRNA-302 awas significantly lower(P<0.05).The levels of VEGF and bFGF in the early stage of HGC-27 decreased significantly with the increase of tamoxifen dosage(P<0.05).Conclusion Tamoxifen can effectively increase the expression of miRNA-302 aand decrease the level of Mcl-1 siRNA in gastric cancer HGC-27 cells.