度洛西汀对抑郁症患者血清microRNA和细胞因子水平的影响

Effect of duloxetine on serum micro RNA and cytokine levels in depressive patients

目的探讨度洛西汀对抑郁症患者血清microRNA和细胞因子水平的影响。方法选取某院收治的抑郁症患者50例(抑郁症组),同期健康查体人群50例(对照组),采用real-time PCR及ELISA法检测两组人群血清miR-132、miR-182及细胞因子IL-1、IL-6、TNF-α水平。抑郁症患者给予度洛西汀治疗8周,比较治疗前后患者血清miR-132、miR-182及细胞因子水平有无差异。同时评价患者临床疗效,并分析临床疗效与血清miR-132、miR-182及细胞因子的相关性。结果 50例患者完成了8周治疗,其中痊愈22例,显效16例,好转8例,无效4例,显效率(痊愈+显效)为76.0%。抑郁症组患者血清miR-132、miR-182、IL-1、IL-6和TNF-α均显著高于对照组,且差异具有统计学意义(P<0.05);抑郁症组治疗后血清miR-132、miR-182和细胞因子IL-1、IL-6、TNF-α均显著降低(P<0.05)。分别以治疗前血清miR-132、miR-182、 IL-1、IL-6和TNF-α为预测度洛西汀显效的敏感性分别为78.2%、71.6%、75.3%、68.4%和66.9%,特异性分别为69.5%、65.8%、60.7%、66.5%和70.3%。诊断的ROC曲线下面积分别为0.73、0.68、0.66、0.64和0.69。结论抑郁症患者血清miR-132、miR-182、IL-1、IL-6和TNF-α显著高于健康人群。给予度洛西汀治疗后上述指标显著降低,并可作为判断度洛西汀疗效的生物学指标。

Objective To investigate the effect of duloxetine on serum microRNA and cytokines in patients with depression.Methods 50 patients with depression in our hospital were selected as depression group and 50 healthy people as control group.Serum levels of microRNA-132,microRNA-182 and cytokines IL-1,IL-6 and TNF-alpha were detected by qPCR and ELISA.Patients with depression were treated with duloxetine for 8 weeks.The serum levels of mir-132,mir-182 and cytokines were compared before and after treatment.At the same time,the clinical efficacy of patients was evaluated,and the correlation between clinical efficacy and serum microRNA-132,microRNA-182 and cytokines was analyzed.Results Fifty patients completed 8 weeks of treatment,of which 22 were cured,16 were markedly effective,8 were improved,6 were ineffective,and the marked efficiency(recovery+marked effect)was 76.0%.The levels of serum microRNA-132,microRNA-182,IL-1,IL-6 and TNF-alpha in depression group were significantly higher than those in control group(P<0.05),and the levels of serum microRNA-132,microRNA-182 and cytokines IL-1,IL-6 and TNF-alpha in depression group were significantly lower after treatment(P<0.05).Serum levels of mir-132,mir-182,IL-1,IL-6 and TNF-alpha before treatment were 78.2%,71.6%,75.3%,68.4%and 66.9%,respectively.Specificity was 69.5%,65.8%,60.7%,66.5%and 70.3%,respectively.The areas under ROC curve were 0.73,0.68,0.66,0.64 and 0.69,respectively.Conclusion The serum levels of microRNA-132,microRNA-182,IL-1,IL-6 and TNF-alpha in patients with depression are significantly higher than those in healthy people.After treatment with duloxetine,the above indicators were significantly reduced,and could be used as a biological index to judge the efficacy of duloxetine.