全自动酶免分析仪检测HIV抗体待复检标本确证结果分析

Automated enzyme-free analyzer for detection of HIV antibody to be retested

目的对ADCC ELISA 500全自动酶免分析仪检测HIV抗体待复检标本的确证结果进行分析,以探讨试验"灰区"样本的正确处理,从而避免漏报、误报不良医疗事件的发生。方法选择某综合医院2018年12月~2019年6月送检血清标本,采用ADCC ELISA 500全自动酶免分析仪进行HIV抗体初筛,对检测待复检标本送到确证实验室经蛋白印迹试验(WB)确证,并对其结果统计分析。结果共初筛出64份待复检样本,其中50份初筛有反应样本经WB确认均为阳性(100.0%),14份"灰区"样本经确认有6份为不确定,8份为阴性。50份确证阳性样本中,GP160条带出现频率最高(100.0%),P55出现频率最低(48.0%);6份不确定样本中,GP160条带出现频率为100.0%。结论全自动酶免分析仪检测HIV抗体标本S/CO值在"灰区"附近时,S/CO值大小不能有效判断结果,要及时送检确证实验室进行WB确证。

Objective To analyze the confirmatory results of the ADCC ELISA 500 automatic enzyme-free analyzer for the detection of HIV antibody to be retested,in order to investigate the correct treatment of the“grey area”sample,so as to avoid false negatives and false positives.Methods some serum samples of a general hospital were selected from December 2018 to June 2019.The ADCC ELISA 500 automatic enzyme-free analyzer was used for primary screening of HIV antibodies.The samples to be retested were sent to the laboratory for confirmation by Western Blot test(WB),and then made a confirmation and statistical analysis of the results.Results A total of 64 samples to be re-examined were screened out.Among them,50 samples of the preliminary screening were positive by WB(100.0%),and 14 samples of“ash area”were confirmed to be 6 indeterminate,8 were negative.Of the 50 positive samples,the gp160 band had the highest frequency(100.0%)and the p55 had the lowest frequency(48.0%);in 6 indeterminate samples,the gp160 band appeared at 100.0%.Conclusion When the S/CO value of the HIV antibody specimen is detected near the“grey area”by the automatic enzyme-free analyzer,the S/CO value cannot be judged effectively.It is necessary to promptly confirm the“grey area”and positive results by Western blotting test in laboratory.