调控蓝色大麦花青素合成代谢的MYC基因克隆及序列分析

Cloning and Sequence Analysis of MYC Gene Regulating Anthocyanin Biosynthesis in the Hordeum vulgare L.

本研究的目的是利用PCR方法克隆了大麦中MYC转录因子HvMYC1。研究结果表明:该基因全长1 668 bp,编码氨基酸555个,分子量为61 333.71,等电点为8.47,有两个属于bHLH超级因家族的保守区,该蛋白具有亲水性,二级结构以α-螺旋(40.90%)和无规则卷曲(41.44%)为主要部分,Hv MYC1蛋白质不通过跨膜区,在膜外进行作用。蛋白系统进化分析表明,HvMYC1基因与小麦、矮牵牛、水稻等物种中调控花青素合成MYC转录因子同源。本研究为解析蓝粒大麦中调控花青素合成代谢基因分子遗传机理提供科学依据,为大麦花青素的继续深入研究提供参考。

The aim of this study is to clone the transcription factor HvMYC1 of MYC gene in Barley by PCR. The results showed this gene is 1 668 bp long, encoding 555 amino acids, with a molecular mass of 61 333.71 and isoelectric point of 8.47, have two conserved regions belonging to the bHLH super-gene family, protein has certain hydrophilicity, the second-level structure is mainly composed of alpha helix and irregularly coiled as the most large structure of protein, transmembrane region analysis suggested that the protein might not have a trans-membrane region, but mainly acted outside the membrane.Evolutionary analysis shows: the HvMYC1 gene is closely related to the regulatory genes of maize, rice and Arabidopsis thaliana. This study provides a theoretical basis for the analysis of molecular genetic mechanism of anthocyanin biosynthesis genes in Hordeum vulgare L.and for the better utilization of anthocyanin.