小黑麦中调控花青素合成代谢的MYC基因克隆及序列分析

Cloning and Sequence Analysis of MYC Gene Regulating Anthocyanin Biosynthesis in Triticale

为了研究蓝粒小黑麦糊粉层中控制花青素合成的关键基因BcMYC1。本研究使用PCR方法克隆小黑麦中MYC转录因子BcMYC1。结果表明,小黑麦BcMYC1基因全长1683 bp,编码的氨基酸560个,分子量61870 Da,等电点4.71,有两个属于bHLH超级因家族的保守区,该蛋白具有亲水性,二级结构以α-螺旋(39.64%)和不规则盘绕(43.93%)为主要的部分,BcMYC1蛋白质不通过跨膜区,在膜外进行作用。蛋白系统进化分析表明,小黑麦的BcMYC1基因与小麦、矮牵牛、水稻等物种MYC调节基因的亲缘关系很高。本研究关于小黑麦中调控花青素合成代谢BcMYC1基因分子遗传机理,为小黑麦的花青素的研究提供参考作用。

In order to study the key gene BcMYC1 of anthocyanin synthesis in the aleurone layer of small blue triticale.In this paper,MYC transcription factor BcMYC1 in triticale was cloned by PCR.The results showed that the length of BcMYC1 gene in triticale was 1683 bp,the encoding amino acid was 560,and the molecular weight was 61870 Da,isoelectric point 4.71.there are two conserved regions of bHLH superfamily.The protein is hydrophilic.The secondary structure is mainly composed ofα-helix(39.64%)and irregular coiling(43.93%)as the main part of BcMYC1 protein acting outside the membrane without passing through the transmembrane region.The phylogenetic analysis showed that the BcMYC1 gene of triticale is closely related to the MYC regulatory gene of wheat,petunia,rice and other species.The molecular genetic mechanism of BcMYC1 gene regulating anthocyanin biosynthesis in triticale was studied in this paper,which provides a reference for the study of anthocyanins in triticale.