42份早熟禾种质资源的SSR遗传多样性分析

SSR Genetic Diversity Analysis of 42 Poa L. Germplasm Resources

本研究基于SSR分子标记,对来自青海和西藏的42份早熟禾种质资源进行遗传多样性分析,以了解两个来源地的早熟禾资源遗传差异及遗传背景。用梯度PCR仪筛选出11对适合早熟禾SSR-PCR引物及其退火温度;结果显示,11对引物对42份材料共扩增出52条谱带,其中多态性谱带为36条,多态率为69.23%;42份早熟禾材料遗传相似系数范围为0.000~0.981,遗传距离范围为0.000~0.291,说明具有较高的遗传多样性。UPGMA聚类分析显示,在阈值约为0.83处,将42份早熟禾材料分为三大类,聚类结果表明材料来源与地理位置、生境之间具有一定的相关性。本研究结果得到具有较高遗传多样性的早熟禾种质资源,可以为早熟禾核心种质构建提供一定的理论基础。

This study analyzed the genetic diversity of 42 germplasm resources from Qinghai and Tibet based on SSR molecular marker,in order to understand the genetic differences and genetic background of different sources.The results showed that:11 pairs of SSR-PCR primers suitable for prematurity and their annealing temperatures were screened by gradient PCR,and 52 bands were amplified by 11 pairs of primers to 42 materials,of which 36 were polymorphisms.The polymorphism rate is 69.23%;the genetic similarity coefficient of 42 precocious mulberry materials ranged from 0.000~0.981 and the genetic distance range was from 0.000 to 0.291,indicating that there was a high genetic diversity.UPGMA clustering showed that at a threshold of about 0.83,42 precocious grapefruit materials were divided into three categories.The clustering showed that the material originated from geographical location andthere was a certain correlation between habitats.The results of this study showed that the germplasm resources of Poa L.had high genetic diversity,which could provide a theoretical basis for the construction of the core germplasm of Poa L.