miR-663对PG-LH7和PG-BE1细胞生长增殖和迁移侵袭的影响

Effect of miR-663 on the Growth Proliferation Migration and Invasion of PG-LH7 and PG-BE1 Cells

目的:探讨microRNA-663(miR-663)对人肺巨细胞癌的低转移亚系PG-LH7和高转移亚系PG-BE1生长增殖、迁移和侵袭能力的影响。方法:实时定量RT-PCR检测A549细胞,PG-LH7细胞、PG-BE1细胞、miR-663ASO转染的PG-BE1细胞和miR-663mimics转染的PG-LH7细胞中miR-663表达水平;利用细胞计数、集落形成实验和CCK8法检测miR-663ASO或miR-663mimics转染后的PG-BE1细胞和PG-LH7细胞增殖;Transwell小室检测miR-663ASO或miR-663mimics转染后的PG-BE1细胞和PG-LH7细胞的侵袭和迁移能力。结果:miR-663在PG-LH7细胞中的表达低于A549细胞中的表达水平(P<0.05),miR-663在PG-BE1细胞中的表达高于A549细胞中的表达水平(P<0.05);miR-663mimics转染可以使miR-663在PG-LH7的表达水平增高(P<0.05),miR-663ASO转染可以使miR-663在PG-BE1的表达水平明显降低(P<0.05);miR-663mimics能够有效的促进PG-LH7细胞的增殖(P<0.05)、侵袭迁移能力(P<0.01);miR-663ASO能够有效的抑制PG-BE1细胞的增殖(P<0.05)、侵袭迁移能力(P<0.01)。结论:miR-663过表达能促进人肺巨细胞癌的低转移亚系PG-LH7的增殖、侵袭和迁移能力,而抑制miR-663表达抑制高转移亚系PG-BE1的生长增殖、迁移和侵袭能力。

Objective:To explore the modulating effect of the microRNA-663(miR-663)on the proliferation ability and invasive ability in human lung cancer cells PG-LH7 and PG-BE1.Methods:Real time quantitative RT-PCR was used to detect the expression of miR-663 in A549 cells,PG-LH7 cells,PG-BE1 cells,PG-BE1 cells transfected by mir-663ASO and PG-LH7 cells transfected by miR-663mimics;cell count,colony forming assay and CCK8 method were used to detect the proliferation of PG-BE1 cells and PG-LH7 cells transfected by miR-663ASO or miR-663mimics;Transwell cell was used to detect the expression of miR-663ASO or miR-663mimics after transfection The invasion and migration ability of PG-BE1 cells and PG-LH7 cells.Results:The expression level of miR-663 in PG-LH7 cells was lower than that in A549 cells(P<0.05),the expression level of miR-663 in PG-BE1 cells was higher than that in A549 cells(P<0.05),the expression level of miR-663 in PG-LH7 was increased by miR-663mimics transfection(P<0.05),the expression level of miR-663 in PG-BE1 was significantly decreased by miR-663ASO transfection(P<0.05),and the expression level of miR-663mimics was effective MiR-663ASO can effectively inhibit the proliferation and invasion of PG-BE1 cells(P<0.05).Conclusion:Overexpression of miR-663 can promote the proliferation,invasion and migration of PG-LH7,a low metastasis sub line of human lung cancer,while inhibition of miR-663 can inhibit the growth,proliferation,migration and invasion of PG-BE1,a high metastasis sub line.