血清抗bFGF自身抗体在活动期自身免疫性疾病中的检测价值分析

Analysis of Detection Value of Serum Anti-b FGF Autoantibodies in Active Autoimmune Aiseases

目的:探讨活动期自身免疫性疾病患者血清抗碱性成纤维细胞生长因子(bFGF)自身抗体表达意义。方法:回顾性分析139例活动期自身免疫性疾病患者临床资料,并将46例同期入院体检的健康人群作为对照,比较不同患病类型、肾损害情况下患者血清抗bFGF-免疫球蛋白(Ig)G抗体与抗bFGF-IgM抗体水平差异,分别以Pearson相关系数模型、受试者工作特征(ROC)曲线,分析上述指标与尿白蛋白排泄率(UAER)的相关性及其对肾损害的预测效能。结果:活动期系统性红斑狼疮(SLE)、干燥综合征(SS)、类风湿性关节炎(RA)、其他类型自身免疫性疾病患者血清抗bFGF-IgG抗体、抗bFGF-IgM抗体水平均明显高于健康人群,且RA患者明显高于SLE患者(P<0.05)。活动期自身免疫性疾病肾损害患者血清抗bFGF-IgG抗体、抗bFGF-IgM抗体及UAER水平均明显高于非肾损害患者(P<0.05)。活动期自身免疫性疾病患者血清抗bFGF-IgG抗体、抗bFGF-IgM抗体水平与UAER水平均表现出显著正相关性(r>0,P<0.05)。血清抗bFGF-IgG抗体、抗bFGF-IgM抗体预测活动期自身免疫性疾病患者肾损害的ROC曲线下面积(AUC)比较无统计学意义(P>0.05),其预测敏感度分为别83.33%、91.67%,特异度分别为64.08%、61.17%,cut-off值分别为208.01×10^-3、224.79×10^-3。结论:活动期自身免疫性疾病患者血清抗bFGF自身抗体表达显著增高,临床检测可为病情评估提供参考,并为病理机制研究奠定基础。

Objective:To investigate the expression significance of serum anti-basic fibroblast growth factor(bFGF)autoantibodies in patients with active autoimmune diseases.Methods:The clinical data of 139 patients with active autoimmune diseases were retrospectively analyzed.46 healthy subjects who were admitted to the hospital for physical examination at the same time were used as control group.The levels of serum anti-bFGF-immunoglobulin(Ig)G antibody and anti-bFGF-IgM antibody were compared among patients with different types of diseases and kidney damage,and the correlation between the above indicators and urinary albumin excretion rate(UAER)and its predictive value on kidney damage were analyzed by Pearson correlation coefficient model and receiver operating characteristic(ROC)curve respectively.Results:The levels of serum anti-bFGF-IgG antibody and anti-bFGF-IgM antibody in patients with active systemic lupus erythematosus(SLE),Sjogren's syndrome(SS),rheumatoid arthritis(RA)and other types of autoimmune diseases were significantly higher than those of healthy subjects,and the levels in RA patients were significantly higher than those in SLE patients(P<0.05).The levels of serum anti-bFGF-IgG antibody,anti-bFGF-IgM antibody and UAER in patients with autoimmune diseases and kidney damage were significantly higher than those of patients with non-kidney damage(P<0.05).Levels of serum anti-bFGF-IgG antibody and anti-bFGF-IgM antibody in patients with active autoimmune diseases showed a significant positive correlation with UAER level(r>0,P<0.05).There was nos ignificant difference in the area under ROC curve(AUC)of serum anti-bFGF-IgG antibody and anti-bFGF-IgM antibody in predicting kidney damage in patients with active autoimmune diseases(P>0.05),and the prediction sensitivities were 83.33%and 91.67%,ans the specificities were 64.08%and 61.17%,and the cut-off values were 208.01×10^-3 and 224.79×10^-3 respectively.Conclusion:The expressions of anti-bFGF autoantibodies are significantly increased in patients with active autoimmune diseases.Clinical testing can provide a reference for disease assessment and lay a foundation for pathological mechanism research.