普拉梭菌干预对溃疡性结肠炎小鼠免疫应答、肠道菌群、肠黏膜屏障的影响

Effects of Faecalibacterium prausnitzii intervention on immune response,intestinal flora and intestinal mucosal barrier of mice with ulcerative colitis

目的研究普拉梭菌干预对溃疡性结肠炎(UC)小鼠免疫应答、肠道菌群、肠黏膜屏障的影响。方法C57BL/6J小鼠随机分为对照组、UC组、普拉梭菌组,后2组采用三硝基苯磺酸灌肠的方法建立UC模型,普拉梭菌组给予普拉梭菌的菌液灌胃干预。干预7 d天后,比较3组间免疫应答、肠道菌群、肠黏膜屏障的差异。结果UC组血清中白介素-10(IL-10)、转化生长因子-β1(TGF-β1)的含量及肠黏膜中叉头框P3(Foxp3)、闭锁小带蛋白-1(ZO-1)、闭锁蛋白(occludin)、密封蛋白-1(claudin-1)、密封蛋白-2(claudin-2)的表达量及粪便中双歧杆菌、乳酸杆菌的数目明显低于对照组,血清中白介素-17(IL-17)、二胺氧化酶(DAO)、D-乳酸(D-LA)的含量及肠黏膜中视黄酸相关孤儿核受体γt(RORγt)的表达量及粪便中肠杆菌、肠球菌的数目明显高于对照组(P<0.05);普拉梭菌组血清中IL-10、TGF-β1的含量及肠黏膜中Foxp3、ZO-1、occludin、claudin-1、claudin-2的表达量及粪便中双歧杆菌、乳酸杆菌的数目明显高于UC组,血清中IL-17、DAO、D-LA的含量及肠黏膜中RORγt的表达量及粪便中肠杆菌、肠球菌的数目明显低于UC组(P<0.05)。结论普拉梭菌用于UC小鼠的干预能够改善Th17/Treg免疫应答、肠道菌群及肠黏膜屏障。

Objective To study the effects of Faecalibacterium prausnitzii intervention on immune response,intestinal flora and intestinal mucosal barrier of mice with ulcerative colitis(UC).Methods C57BL/6J mice were randomly divided into control group,UC group and Faecalibacterium prausnitzii group,the latter two groups were made into UC models by trinitrobenzene sulfonic acid enema and F.prausnitzii group were given intragastric administration of F.prausnitzii solution for intervention.The differences in immune response,intestinal flora,and intestinal mucosal barrier were compared among the three groups after 7 days of intervention.Results The interleukin-10(IL-10)and transforming growth factor-β1(TGF-β1)contents in serum,the fork head box P3(Foxp3),zonula occludens-1(ZO-1),occludin,claudin-1 and claudin-2 expression in intestinal mucosa as well as the number of bifidobacterium and lactobacillus in feces of the UC group were significantly lower than those of the control group whereas the interleukin-17(IL-17),diamine oxidase(DAO)and D-lactic acid(D-LA)contents in serum,the retinoid-related orphan nuclear receptorγt(RORγt)expression in intestinal mucosa as well as the number of enterobacter and enterococcus in feces were significantly higher than those of the control group(P<0.05);IL-10 and TGF-β1 contents in serum,Foxp3,ZO-1,occludin,claudin-1 and claudin-2 expression in intestinal mucosa as well as the number of bifidobacterium and lactobacillus in feces of the F.prausnitzii group were significantly higher than those of the UC group whereas IL-17,DAO and D-LA contents in serum,RORγt expression in intestinal mucosa as well as the number of enterobacter and enterococcus in feces were significantly lower than those of the UC group(P<0.05).Conclusion Faecalibacterium prausnitzii intervention can improve the Th17/Treg immune response,intestinal flora and intestinal mucosal barrier in UC mice.