和厚朴酚对人皮肤鳞癌细胞A431增殖凋亡作用及可能的机制分析

The effect of Honokial on proliferation and apoptosis of human cutaneous squamous cell carcinoma A431 cells and possible mechanism

目的:研究和厚朴酚(Honokial,HNK)对人皮肤鳞癌细胞A431增殖凋亡作用及可能的机制分析。方法:采用CCK8实验方法检测和厚朴酚对A431细胞增殖的影响;Western blot定量分析和厚朴酚对Bcl-2和Bad蛋白表达的影响;Western blot定分析和厚朴酚对A431细胞中p53蛋白表达的影响;利用p53过表达腺病毒,并联合应用药物,通过CCK8和Western blot方法分析和厚朴酚对细胞增殖和凋亡的影响。结果:和厚朴酚以浓度和时间依赖性地抑制A431细胞增殖,并诱导其凋亡,上升Bad蛋白水平,下调Bcl-2蛋白水平;和厚朴酚可上调A431细胞中p53蛋白表达水平;加入p53过表达腺病毒可增强和厚朴酚的作用,抑制细胞增殖,促进细胞凋亡。结论:和厚朴酚能够抑制人皮肤鳞癌细胞A431的增殖并诱导凋亡,其机制可能与p53信号通路有关。

To investigate the effect of Honokial and the possible mechanism on human cutaneous squamous cell carcinoma A431 cells.Methods:CCK8 assay was used to test the proliferation inhibitory effect of Honokial on cells.Quantitative Western blot was used to analyse the expressions of Bad and Bcl-2.Western blot was conducted to analyse the level of p53.Exogenous adenoviruses of p53 combined with Honokial were added to analyse the proliferation inhibitory and apoptosis effect on cells by Western blot assay.Results:Honokial could inhibit the growth of human cutaneous squamous cell carcinoma A431 cells in a time and concentration dependent way and induce apoptosis.Western blot assay showed Honokial up-regulated the level of p53.Western blot results showed that exogenous adenoviruses of p53 could enhance proliferation inhibitory and apoptosis effect induced by Honokial.Conclusion:Honokial could inhibit the proliferation and induce apoptosis on A431 cells,which may be mediated by p53 signal pathway.