西黄胶囊对大鼠放射性口腔黏膜炎的防护作用

The protection of Xihuang capsules against radiation-induced oral mucositis in rats

目的:探讨西黄胶囊对大鼠放射性口腔黏膜炎的防护作用及相关机制。方法:将54只雌性Wistar大鼠随机分为给药组、单纯照射组和空白对照组。除空白对照组,其他两组均以6 MV X射线左侧颊黏膜单次30 Gy照射制作大鼠放射性口腔黏膜炎模型。从第1天开始至照射后第28天连续灌胃,给药组灌西黄混悬液,单纯照射组和空白对照组灌生理盐水。照射后观察大鼠颊黏膜的变化情况,照射后第3、14、28天处死大鼠观察颊黏膜病理切片,用ELISA法检测血清中白介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)水平,RT-PCR法检测颊黏膜组织中IL-1β和TNF-αmRNA的表达。结果:与单纯照射组比较,给药组大鼠口腔黏膜炎明显减轻;给药组血清中IL-1β和TNF-α水平及颊黏膜组织中IL-1β和TNF-αmRNA表达均较单纯照射组低(P<0.05)。结论:西黄胶囊能够减轻照射大鼠口腔黏膜的炎性反应,其机制可能与抑制炎性因子IL-1β和TNF-α的释放、下调相关基因的表达有关。

Objective:To investigate the protective effect and relative mechanism of Xihuang capsules on radiation-induced oral mucositis in rats.Methods:Totally 54 female Wistar rats were randomly divided into administration group,irradiation alone group and blank control group.Except for the blank control group,the other two groups were modeled with a single dose of 30 Gy in 6 MV X-ray by Varian Trilogy.The administration group rats were given Xihuang suspension for continuous gastric perfusion from the 1st day to the 28th day after irradiation,while the irradiation alone group and the blank control group were given normal saline.The changes of buccal mucosa were evaluated daily after irradiation.The rats were sacrificed on the 3rd,14th and 28th day after irradiation to observe the pathological sections of buccal mucosa.After rats being sacrificed,the levels of interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in serum were measured by ELISA,and IL-1βand TNF-αmRNA expression in buccal mucosa tissue were assayed by RT-PCR.Results:After Xihuang capsules treatment,the inflammatory reaction of buccal mucosal tissue was markedly decreased compared with irradation alone group.Furthermore,the serum levels of IL-1βand TNF-αwere obviously reduced(P<0.05)and the IL-1βand TNF-αmRNA levels in buccal mucosal tissue were also decreased(P<0.05).Conclusion:Xihuang plays a mucoprotective role against radiation-induced oral mucositis in rats and the down-expression of IL-1βand TNF-αmay be involved.