肠道菌群及炎性因子联合检测在炎症性肠病患者中的诊断价值研究

The diagnostic value of combined detection of gut microbiota and inflammatory cytokines in patient with inflammatory bowel disease

目的探讨肠道菌群及血清炎性因子联合检测在炎症性肠病(inflammatory bowel disease,IBD)中的诊断价值。方法选取2017年6月至2018年5月我院收治的IBD患者174例作为实验对象,根据病情的分期分组,分为活动期组和缓解期组。同时,选取同期在我院进行健康体检者30例作为对照组。分别采集各组新鲜粪便,培养样本并分析肠道菌群。此外,采集各组外周静脉血,采用酶联免疫吸附法(enzyme-linked immuno sorbent assay,ELISA)分别检测各组血清IL-6,TNF-α和hs-CRP表达水平。结果结果表明,各组性别、身高、体重、BMI均无明显差异(P>0.05)。肠道菌群数量检测结果显示,与对照组比较,活动期组和缓解期组肠球菌(EC)、酵母菌(SB)、双歧杆菌(BL)和拟杆菌(BD)数量明显增加,而真杆菌(ES)数量明显减少(P<0.05)。而各组肠杆菌(EMB)数量无明显差异(P>0.05)。同时,与对照组相比,活动期组和缓解期组EC、SB、BD菌群阳性检出率明显增高,而ES菌群明显降低,差异有统计学意义(P<0.05)。而各组EMB和BL菌群阳性检出率无明显差异(P>0.05)。ELISA法检测结果显示,活动期组和缓解期组IL-6,TNF-α和hs-CRP表达水平均较对照组明显增加(P<0.05)。结论 IBD患者肠道菌群和血清炎性因子含量联合检测将有助于IBD患者的诊断及病情严重程度的判断。

Objective To investigate the diagnostic value of gut microbiota and inflammatory cytokines combined detection in patient with inflammatory bowel disease(IBD).Methods 174 patients with IBD from June 2017 to May 2018 were selected as experimental objects in our hospital,which were divided into active stage(AS)group and remission stage(RS)group.Meanwhile,30 healthy subjects were selected as the control group at the same period.Fresh dejection in each group was harvested,then the specimens were cultured and the gut microbiota was analyzed,respectively.Moreover,peripheral venous blood of experimental group and control group was collected,and enzyme-linked immuno sorbent assay(ELISA)was employed to determine the expression level of IL-6,TNF-αand hs-CRP.Results The results showed that there was no statistical difference in gender,height,weight and BMI between experimental group and control group(P>0.05).The number of gut microbiota including EC,SB,BL and BD was obviously increased,while the number of ES was significantly decreased in AS group and RS group when compared to control group(P<0.05).There was no significant differences in the number of EMB among groups(P>0.05).Positive detection rate of gut microbiota consisting of EC,SB and BD was obviously increased,while which in ES was significantly decreased in AS group and RS group compared with control group(P<0.05).There was no overtly differences in the positive detection rate of EMB and BL among groups(P>0.05).The expression of serum IL-6,TNF-αand hs-CRP indicated by ELISA assay was obviously increased in AS group and RS group compared to that of in control group(P<0.05).Conclusion The combined detection of gut microbiota and inflammatory cytokines is conducive to diagnose and judge the severity of IBD.