基于生物热力学的不同产地柴胡抑菌活性评价

Antibacterial Activity Evaluation of Bupleurum chinensis from Different Habitats Based on Biothermodynamics

目的:分析不同产地柴胡品质差异,为探索柴胡抑菌活性成分提供依据。方法:以HPLC法测定不同产地柴胡中柴胡皂苷a和柴胡皂苷d的含量。以微量热法测定不同产地柴胡对金黄色葡萄球菌的生长代谢曲线,得出热力学参数,并进行相关分析。结果:不同产地柴胡热动力学差异较大,其抑制率在7.14%~53.06%,说明不同程度抑制了金黄色葡萄球菌的生长。其中,山西和陕西柴胡抑菌活性优于河北柴胡。柴胡皂苷含量最高的山西柴胡S3抑菌作用最强。利用SPSS软件对色谱峰峰面积与抑制率进行Spearman双变量相关分析,筛选相关系数大于0.5的色谱峰,柴胡皂苷a、d的相关系数分别为0.783与0.717,说明柴胡皂苷a和柴胡皂苷d与柴胡抑菌作用存在着密切的关联性。结论:微量热法可以快速准确地测定不同产地柴胡的抑菌效果。化学成分检测结合生物热力学测定,可以评价柴胡的生物质量。

Objective:The purpose of this study is to investigate the quality of Bupleurum chinensis from different habitats and provide theoretical basis for antibacterial active ingredient of Bupleurum chinensis.Methods:The contents of saikosaponin a and saikosaponin d in Bupleurum from different habitats were determined by high-performance liquid chromatography(HPLC)method.The growth thremogram curves of Staphylococcus aureus treated with Bupleurum from different habitats were determined by microcalorimetry,and the biothermodynamics parameters in the curve were performed by correlation analysis.Results:The results showed different about thermodynamic parameters among the difference Bupleurum area,the inhibition rate changed between 7.14%and 53.06%,which indicated that there was different degrees of inhibitory effects on the growth of Staphylococcus aureus,and Bupleurum from shanxi and shannxi showed stronger bacteriostatic activity than hebei.The highest content of saikosaponin in Bupleurum from Shanxi S3 has the strongest antibacterial effect.Spearman bivariate correlation analysis was employed to investigate the relationship between the peak areas and the inhibition by SPSS software,the chromatographic peaks with correlation coefficient greater than 0.5 were identified,the correlation coefficients of saikosaponins a and d were 0.783 and 0.717,which indicated that there was a close relation between saikosaponins a and saikosaponins d in Bupleurum and the antibacterial activity of Bupleurum chinense.Conclusion:Microcalorimetry can detect the antibacterial effect of Bupleurum chinensis from different habitats quickly and accurately.Chemical composition detection combined with biothermodynamic can be used to evaluate the biogical quality of Bupleurum chinensis.