聚葡萄糖润肠通便功能及其有效剂量研究

Study on the Laxative Function and Effective Dose of Polydextrose

为研究聚葡萄糖润肠通便作用的效果及有效剂量,将120只昆明雄性小鼠,随机分为两个实验组,一组进行小肠运动实验,一组进行排便实验,每个实验组又随机分为空白对照组、模型对照组、聚葡萄糖干预组(低、中、高3个剂量),低、中、高剂量分别为0.5、1.0、3.0 g/kg,连续干预14 d后,利用盐酸洛哌丁胺建立小鼠便秘模型,测定小肠墨汁推进率、首例排黑便时间、6 h内排黑便例数和粪便重量。结果表明,与模型对照组相比,聚葡萄糖低、中、高剂量组小肠墨汁推进率有增加趋势,仅高剂量组差异具有统计学意义(P<0.05);聚葡萄糖低、中、高剂量组首例排黑便时间、6 h内排黑便例数均无显著差异(P>0.05);低、中剂量组的粪便重量与模型对照组无差异,但高剂量组粪便重量较模型对照组极显著升高(P<0.01)。聚葡萄糖具有润肠通便的作用,其有效剂量为3.0 g/kg。

In order to study the effect and effective dose of polydextrose on the laxative function,120 Kunming male mice were randomly divided into two trail groups,one for small intestine movement experiment and the other for defecation experiment.Each trail group was randomly divided into blank control group,model control group and low,middle and high polydextrose groups.Low,medium and high dose concentrations were 0.5,1.0 and 3.0 g/kg,respectively.After 14 days of intervention,loperamide was used to establish the constipation model of mice,then the intestinal ink propulsion rate,first black stool time,fecal number and fecal weight within 6 h were measured.The results showed that,compared with the model control group,the intestinal propulsion rate of the low,middle and high dose polydextrose group had an increasing trend,but the difference was statistically significant only in the high dose group(P<0.05);there was no significant difference in the first defecation time and the number of fecal particles in the low,middle and high dose polydextrose groups(P>0.05).There was no difference in fecal weight between the low and meddle dose polydextrose groups and the model control group,but the fecal weight of the high dose polydextrose group was extremely significantly higher than that of the model control group(P<0.01).Therefore,high dose of polydextrose had a laxative effect,the effective dose was 3.0 g/kg.