基于HS-SPME-GC/MS指纹图谱及化学计量学的野坝子蜂蜜鉴别

Research on Elsholtzia rugulosa Hems L. Honey via HS-SPME-GC/MS Fingerprint and Chemometrics Analysis

为了实现野坝子蜂蜜的有效鉴别,收集了代表性产地大姚-滇中北部冷凉区、弥勒-滇中部半干旱区野坝子蜂蜜以及普通蜂蜜样品,采用HS-SPME-GC/MS分析,结合指纹图谱、主成分分析(PCA)、欧氏距离以及聚类分析等数据分析手段,均能实现对野坝子蜂蜜与普通蜂蜜的准确判别。同时,在指纹图谱所指认的15个共有峰基础上,筛选出反式氧化芳樟醇、顺式氧化芳樟醇、壬酸乙酯、脱氢芳樟醇和苯乙醇等5个峰峰面积构建了3个Fisher函数,能够快速有效地实现野坝子蜂蜜与普通蜂蜜的鉴别。此外,为了实现不同产地野坝子蜂蜜的识别及质量控制,对两个代表性野坝子蜂蜜产地野坝子蜂蜜成分,采用F检验进行分析,表明由于产地特征差异2个代表性产地野坝子蜂蜜成分差异明显,其中丁香醇C、间苯二甲醛、丁香醇D、月桂酸乙酯、α-异氟尔酮、苯甲酸乙酯、乙酸苯乙酯、异丁醇、2-甲基丁醇等9个成分可作为2个产地野坝子蜂蜜之间的主要差异成分。本研究为野坝子蜂蜜的真伪鉴别以及不同产地野坝子蜂蜜产地识别评价提供技术支持。

In order to realize the effective identification of the Elsholtzia rugulosa Hems L. honey,the representative producing areas Elsholtzia rugulosa Hems L. and the common honey samples were collected,HS-SPME-GC/MS analysis combined with fingerprint,principal component analysis(PCA),euclidean distance and cluster analysis was used to achieve accurate discrimination between Elsholtzia rugulosa Hems L. honey and common honey. At the same time,based on the 15 common peaks identified by the fingerprint,five peaks such as trans-oxylated linalool,cis-arylene oxide,ethyl phthalate,dehydrolinol and phenylethyl alcohol were screened out. The three Fisher functions were constructed by the peak area which could quickly and effectively identify the Elsholtzia rugulosa Hems L. honey and common honey. In addition,in order to realize the identification and quality control of the Elsholtzia rugulosa Hems L. honey from different producing areas,the honey difference components of the two representative Elsholtzia rugulosa Hems L. honey were analyzed by F test,which indicated that the honey components of the two representative producing areas were significantly different due to the difference of origin characteristics. Among which syringol C,isophthalaldehyde,syringol D,ethyl laurate,α-isophorone,ethyl benzoate,phenylethyl acetate,isobutanol,2-methylbutanol,etc,nine ingredients could be used as the main difference between the honey of the two producing areas. This study provides technical support for the identification of the authenticity of the Elsholtzia rugulosa Hems L. honey and the identification and evaluation of the honey producing areas.