无热量超短波对脑缺血再灌注损伤大鼠早期CoQ10和C1GALT1C1表达的影响

Effects of non-caloric ultrashort wave on the expression of CoQ10 and C1GALT1C1 in rats with cerebral ischemia reperfusion injury

目的:探讨脑缺血再灌注损伤大鼠在不同时间点予以无热量超短波(ultrashort wave,USW)治疗后脑内辅酶Q10(coenzyme Q10,CoQ10)、β1,3-半乳糖基转移酶-特异性伴侣1(β1,3-galactosyl transferase specific chaperone 1,C1GALT1C1)表达水平的变化趋势及其对缺血性脑损伤的保护机制。方法:50只Sprague-Dawley大鼠随机分为5组,每组10只。1组是作为对照的假手术组,线栓插入深度为1 cm;其余4组为实验组(分别为模型1 d组、USW1 d组、模型3 d组、USW3 d组),线栓插入深度为18 mm,2 h后予以再灌注。4个实验组中,每组随机选取5只大鼠行盐酸2,3,5-三苯基四氮(2,3,5-triphenyltetrazoliumchloride,TTC)染色,其余5只大鼠行蛋白质印迹法和real-time PCR检测,观察比较各组大鼠脑梗死体积百分比值和缺血侧大脑中CoQ10和C1GALT1C1的相对表达量。结果:TTC染色后所得脑梗死体积百分比值中,假手术组未见脑梗死,比值为0;实验组随着病程延长和USW治疗呈下降趋势,差异均有统计学意义(均P<0.05)。蛋白质印迹法和real-time PCR检测显示:假手术组CoQ10相对表达量最高,但实验组CoQ10相对表达量随着病程延长和USW治疗呈上升趋势,差异均有统计学意义(均P<0.05);假手术组C1GALT1C1的相对表达量最低,但实验组C1GALT1C1的相对表达量随着病程延长和USW治疗呈下降趋势,差异均有统计学意义(均P<0.05)。结论:无热量USW治疗脑缺血再灌注损伤大鼠,可能通过上调CoQ10表达及下调C1GALT1C1表达而发挥保护作用。

Objective:To examine the changes of coenzyme Q10(CoQ10)andβ1,3-galactosyl transferase specific chaperone 1(C1GALT1C1)in brain of rats with ischemic injury at different time points and to explore the protective mechanism of ultrashort wave(USW)on ischemic brain injury.Methods:Fifty SD rats were randomly divided into 5 groups(n=10 per group):a sham group(control group)and 4 experimental group(ischemia for 2 h).The 4 experimental groups were set as a model 1 d group,a USW 1 d group,a model 3 d group and a USW 3 d group,respectively.Five rats were randomly selected for 2,3,5-triphenyltetrazoliumchloride(TTC)staining in each experimental group,and the remaining 5 rats were subjected to Western blotting and real-time PCR.The percentage of cerebral infarction volume and the relative expression level of CoQ10 and C1GALT1C1 in the brain were examined and compared.Results:The infarct volume percentage after TTC staining was zero in the sham group.With the progress of disease and USW therapy,the infarct volume percentage was decreased in the experimental groups(all P<0.05);Western blotting and real-time PCR showed that the relative expression level of CoQ10 in the sham group was the highest,while in the experimental groups,the content of CoQ10 showed a upward trend with the extension of disease and USW therapy,with significant difference(all P<0.05).The relative expression level of C1GALT1C1 in the sham group was the lowest,but in the experimental groups,they showed a downward trend with the extension of disease and USW therapy,with significant difference(all P<0.05).Conclusion:Non-caloric USW therapy may upregulate the expression of CoQ10 to suppress the expression of C1GALT1C1 in rats,leading to alleviating cerebral ischemic reperfusion injury.