血载脂蛋白E与血浆同型半胱氨酸水平和痴呆患者病情严重程度及分类的关系

Relationship between serum apolipoprotein E,Hcy levels and the disease severity,types of dementia patients

目的分析血载脂蛋白E(ApoE)基因型和血浆同型半胱氨酸(Hcy)水平与痴呆患者病情严重程度及分类的关系。方法选择被确诊的88例痴呆患者为研究组,选取同期进行体检的88例健康老年人为对照组。采用PCR-RELP检测ApoE基因分型;采用荧光化学发光法检测Hcy水平;采用Pearson相关性分析ApoE基因分型和Hcy水平与痴呆患者病情严重程度之间的相关性。结果不同严重程度痴呆患者的Hcy水平均显著高于对照组(P<0.05);中度和重度痴呆患者的Hcy水平显著高于轻度痴呆患者(P<0.05),ApoEε4等位基因频率显著高于对照组(P<0.05);不同类型痴呆患者的血清Hcy水平均显著高于对照组(P<0.05),且血管性痴呆(VD)患者的血清Hcy水平显著高于帕金森病痴呆(PDD)和阿尔茨海默病(AD)患者(P<0.05);AD患者的ApoEε3等位基因频率显著高于对照组(P<0.05),AD和VD患者的ApoEε4等位基因频率显著高于对照组和PDD患者(P<0.05);痴呆患者ApoEε4等位基因频率升高和Hcy水平升高与痴呆患者病情严重程度呈显著正相关关系(r=0.223,P<0.001;r=0.748,P<0.001)。结论痴呆患者ApoEε4等位基因频率升高和Hcy水平升高与痴呆患者病情严重程度呈显著正相关关系。

Objective To analyze the relationship between serum apolipoprotein E(ApoE)genotype,plasmahomocysteine(Hcy)levels and the disease severity and types of dementiapatients.Methods A total of 88 dementia patients confirmed in the hospital were enrolled.Another 88 healthy elderly patients who underwent physical examination in the same period were enrolled as control group.ApoE genetic typing was detected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RELP).Hcy level was detected by fluorescence chemiluminescence method.Pearson correlation analysis was performed to analyze correlation between ApoE genetic typing,Hcy level and disease severity of dementia patients.Results The serum Hcy levels of dementia patients with different severity were significantly higher than those of control group(P<0.05).The Hcy levels of patients with moderate and severe dementia were significantly higher than those of patients with mild dementia(P<0.05).The ApoEε4 allele frequency was significant higher than that in control group(P<0.05).The serum Hcy levels of patients with different types of dementia were significantly higher than those of control group(P<0.05).The level of serum Hcy in VD patients was significantly higher than that in PDD and AD patients(P<0.05).The ApoEε3 allele frequency in AD patients was significantly higher than that in control group(P<0.05).The ApoEε4 allele frequency in AD and VD patients was significantly higher than that in control group and PDD patients(P<0.05).The increase of ApoEε4 allele frequency and increase of Hcy level in dementia patients were significantly positively correlated with disease severity of dementia patients(r=0.223,P<0.001.r=0.748,P<0.001).Conclusion The increase of ApoEε4 allele frequency and increase of serum Hcy level are correlated with disease severity of dementia patients.