miR-601靶向调控MMP-17抑制非小细胞肺癌的迁移和侵袭

miR-601 Suppresses Migration and Invasion of Non-small Cell lung Cancer Cells by Targeting MMP-17

目的探讨miR-601对非小细胞肺癌(NSCLC)迁移和侵袭能力的影响并探讨其可能的作用机制。方法RT-qPCR检测NSCLC组织中miR-601的表达,并分析表达水平与癌细胞侵袭和淋巴结转移的相关性。将miR-601 mimics瞬时转染A549细胞或H1299细胞,Transwell实验检测其对两种细胞迁移和侵袭能力的影响。生物信息学预测miR-601相关靶基因,采用双荧光素酶实验进行靶基因验证。检测miR-601的靶基因对A549细胞或H1299细胞迁移及侵袭能力的影响。结果miR-601在NSCLC组织中表达明显降低(P<0.001),且降低水平与癌细胞的浸润(P<0.007)及淋巴结转移(P<0.011)密切相关。瞬时转染miR-601 mimics能明显抑制A549细胞或H1299细胞的迁移及侵袭能力。生物信息学及荧光素酶报告实验提示MMP-17是miR-601的靶基因。MMP-17能促进A549细胞或H1299细胞的迁移和侵袭,但其促进迁移侵袭的能力可被miR-601抑制。结论miR-601通过靶向调控MMP-17而抑制非小细胞肺癌的迁移和侵袭。

Objective To investigate the effects of miR-601 on the migration and invasion of non-small cell lung cancer cells and the underlying mechanism in vitro.Methods RT-qPCR were performed to detect miR-601 expression in NSCLC and adjacent normal tissues,and we analyzed the correlation between the expression level of miR-601 and cancer cell invasion or lymph node metastasis.The A549 or H1299 cells were transfected with miR-601 mimics,and cell migration and invasion abilities were tested by Transwell assay.The bioinformatics was used to predict the target gene of miR-601,and a luciferase reporter assay was used to validate the target gene.We detected the effect of miR-601 target gene on the migration and invasion of A549 or H1299 cells.Results miR-601 expression was down-regulated in NSCLC tissues(P<0.001).miR-601 expression were significantly correlated with the invasion(P<0.007)and lymph node metastasis(P<0.011)of NSCLC.Overexpression of miR-601 mimics in A549 or H1299 cells reduced cell migration and invasion.MMP-17 was a target gene of miR-601.Overexpression of MMP-17 significantly promoted the migration and invasion of A549 or H1299 cells,whereas miR-601 expression significantly inhibited the suppressed cell invasion induced by MMP-17.Conclusion miR-601 suppresses the migration and invasion of NSCLC cells by targeting MMP-17.