不同长牡蛎群体对海水酸化的生理响应差异分析

Analysis of physiological responses in different populations of Crassostrea gigas to seawater acidification

海水酸化暴露可对海洋生物产生多层面的影响。本研究以潮间带野生与潮下带养殖长牡蛎(不同生境背景)的不同组织(鳃、外套膜及消化腺)为研究对象,分析在室内调控p CO2模拟海水酸化暴露条件下,其基础代谢活动、能量代谢以及氧化应激相关指标的变化情况。结果显示:海水酸化暴露后,两种长牡蛎(Crassostrea gigas)的基础代谢过程均受到了一定抑制作用且受影响程度差异明显。潮间带野生与潮下带养殖长牡蛎的关键生理过程(能量代谢及氧化应激)对海水酸化暴露存在不同的响应变化,表明两种长牡蛎应对海水酸化的调节机制可能存在差异。依据PLS-DA分析结果显示,在所有生理指标中,对样本的差异贡献较高(VIP值>1)的指标为:SDH、AST、ATPase、ATP含量、糖原含量、CAT、GST及SOD,表明海水酸化暴露后,在两种长牡蛎的3种组织中上述指标的响应变化程度更大。综合评价分析多个生理指标的整体变化揭示:在海水酸化暴露条件下,潮间带野生长牡蛎比潮下带养殖长牡蛎对海水酸化的生理响应更为剧烈;相比于鳃及消化腺组织,长牡蛎外套膜组织可能受影响更大。

The impact of seawater acidification exposure could pose many effects on marine organisms.In this study,different tissues including gills,mantles,digestive glands of intertidal wild-type and subtidal selectively bred Crassostrea gigas,whose habitats and backgrounds were distinct,were taken as research objects to analyze the changes of basal metabolism,and the related indicators of energy metabolism and oxidative stress under pCO2-driven seawater acidification exposure.The results showed the basal metabolic processes were inhibited,and with distinct differences of intertidal wild-type and subtidal selectively bred oyster under seawater acidification exposure.The physiological processes(energy metabolism and oxidative stress)of these two kinds of C.gigas may have different changes,and the regulating mechanism may be different in response to seawater acidification.According to the PLS-DA analysis,the physiological indicators that contribute significantly to the difference of samples(VIP value>1)are:SDH,AST,ATPase,ATP,glycogen content,CAT,GST and SOD,indicated that the above indicators in three tissues of both oysters was more varied to seawater acidification.Comprehensive analysis of the changes of multiple physiological indicators revealed that intertidal wild-type C.gigas may be more severely affected than subtidal selectively bred C.gigas in physiological response,and the mantle tissues may be more influenced than gill and digestive gland tissues under seawater acidification exposure.