RNA甲基转移酶BCDIN3D的表达与乳腺癌预后的关系

Increased expression of RNA methyltransferase BCDIN3D associated with poor prognosis in breast cancer

目的探讨RNA甲基转移酶BCDIN3D的表达与乳腺癌患者预后的关系。方法选取2013年6月至2015年1月重庆市红十字会医院收治的乳腺癌患者80例,年龄19~65(46.7±4.7)岁;分子分型Luminal A、Luminal B、ERBB2+、Basal-like型分别10、32、20、18例;肿瘤分期T 0~1、T 2、T 3分别9、34、37例;淋巴结分期N 0~1、N 2、N 3分别9、38、33例。均行标准乳腺癌改良根治术,术中获取乳腺癌组织标本,采用免疫组织化学法(IHC)检测乳腺癌组织中BCDIN3D的表达情况。对患者进行随访,统计总生存期(OS)。采用COX比例风险模型分析乳腺癌患者预后的影响因素。结果80例乳腺癌患者中,BCDIN3D阳性表达者30例(37.5%);BCDIN3D阴性表达者50例(62.5%)。BCDIN3D阳性表达者与BCDIN3D阴性表达者间肿瘤分期和淋巴结分期差异有统计学意义(P<0.05),不同年龄、月经状态、分子分型差异均无统计学意义(P>0.05)。中位随访时间为42(23~64)个月,Kaplan-Meier分析结果显示,BCDIN3D阳性表达者、BCDIN3D阴性表达者的中位OS分别为41.2(95%CI:31.2~46.9)、49.5(95%CI:42.1~51.6)个月,BCDIN3D阳性表达者的OS短于BCDIN3D阴性表达者(χ^2=25.241,P<0.001)。COX分析结果显示,肿瘤分期、淋巴结分期、分子分型、BCDIN3D表达是影响乳腺癌患者OS的独立预后因素(P<0.05)。结论BCDIN3D阳性表达与乳腺癌患者预后不良有关。

Objective To investigate the relationship between the expression level of RNA methyltransferase BCDIN3D and the prognosis of breast cancer.Methods Eighty breast cancer patients admitted to Chongqing Red Cross Hospital from June 2013 to January 2015 were enrolled.The patients were 19-65(46.7±4.7)years old,and the molecular classifications of Luminal A,Luminal B,ERBB2+,and Basal-like were 10,32,20,and 18 cases,respectively.Tumor stage of T0-1,T2,T3 were 9,34,and 37 cases,respectively,and lymph node stage of N0-1,N2,and N3 were 9,38,and 33 cases,respectively.All patients underwent standard modified radical mastectomy.Breast cancer tissue samples were obtained during operation.Immunohistochemistry(IHC)was used to detect the expression of BCDIN3D in breast cancer tissues.The overall survival were analyzed by follow up.The Cox proportional hazards model was used to analyze the influencing factors of breast cancer patients'prognosis.Results Of the 80 patients,30(37.5%)were positive for BCDIN3D expression and 50(62.5%)were negative for BCDIN3D expression.There were significant differences in tumor staging and lymph node staging between BCDIN3D positive expression and BCDIN3D negative expression(P<0.05).There were no significant differences in age,menstrual status and molecular typing(P>0.05).The median follow-up time was 42(23-64)months.Kaplan-Meier analysis showed that the median OS of BCDIN3D positive expression and BCDIN3D negative expression were 41.2(95%CI:31.2-46.9)and 49.5(95%CI:42.1-51.6)months,respectively.OS of BCDIN3D positive expression was shorter than BCDIN3D negative expression(χ^2=25.241,P<0.001).Tumor stage,lymph node staging,molecular typing,and BCDIN3D expression were independent prognostic factors for OS in breast cancer patients(P<0.05).Conclusions The positive expression of BCDIN3D is associated with poor prognosis in breast cancer patients.