针刺手法对自发性高血压大鼠胸主动脉BKCa离子通道细胞电生理的调控研究

Regulation of acupuncture manipulation on cell-electrophysiology of BKCa channel in aorta vascular muscle in spontaneously hypertensive rat

[目的]观察针刺手法对自发性高血压大鼠钙激活钾(BKCa)通道电生理特性的影响。[方法]24只16周龄自发性高血压大鼠随机分为模型组、针刺手法组和无手法组,每组8只,8只相同周龄的WKY大鼠作为正常对照组。针刺手法组针刺人迎穴,施用小幅度、120次/min的捻转手法1 min,无手法组进针后不施用手法,留针15 min。采用无创鼠尾测压仪测量血压,利用膜内面向外单细胞膜片钳技术检测BKCa通道的电流、开放时间及开放概率。[结果]模型组、针刺手法组、无手法组的收缩压和舒张压均明显高于正常对照组(P<0.05),针刺手法组在治疗1、2、3、4周血压值显著低于模型组(P<0.05),无手法组仅在治疗1周时收缩压和舒张压均低于模型组(P<0.05);模型组开放概率和开放时间明显低于正常对照组(P<0.05),针刺手法组的开放概率高于模型组(P<0.05);无手法组与模型组比较,差异无统计学意义(P>0.05)。[结论]120次/min的捻转手法作用于人迎穴可影响BKCa通道的电生理特性,其降压作用可能与提高BKCa通道的开放活动有关。

[Objective] To observe the effect of acupuncture manipulation on cell-electrophysiology of calcium-activated potassium channel in aorta vascular muscle in spontaneously hypertensive rats. [Methods] Twenty-four aged 16 weeks spontaneously hypertensive rats(SHR) were randomly divided into the model group,the acupuncture manipulation group and the none acupuncture manipulation group.Eight WKY of the same age rats were used as the normal control group(WKY group). In the acupuncture manipulation group,Renying(ST9) was punctured with twisting manipulation of 120 times per minute for 1 minute. In the non-manipulation group,no manipulation was applied after inserting the needles. The needles were retained for 15 minutes in the two groups. The blood pressure was measured by non-invasive tail manometer,the current,opening time and opening probability of BKCa channel were measured by in-outward single-cell patch clamp technique. [Results] The systolic and diastolic blood pressures of model group,acupuncture manipulation group and nonmanipulation group were significantly higher than those of WKY group(P<0.05). The blood pressure values of acupuncture manipulation group were significantly lower than those of model group at 1,2,3 and 4 weeks after treatment(P<0.05). Systolic blood pressure and diastolic blood pressure in the non-manipulation group were lower than that in the model group only after 1 week of treatment(P<0.05).The open probability and open time of BKCa channel in the model group were significantly lower than those of the normal control group(P<0.05),and the open probability of the acupuncture manipulation group was higher than that of the model group(P<0.05). There was no significant difference between non-manipulation group and model group(P>0.05). [Conclusion] The twisting manipulation of 120 times/min on ST9 can affect the cell-electrophysiology of calcium-activated potassium channel in aorta vascular muscle in spontaneously hypertensive rats. The antihypertensive effect may be related to regulation of the BKCa channel opening activities.