雌激素促进星形胶质细胞系U-87增殖和侵袭

Estrogen promotes proliferation and invasion of astrocyte cell line U-87

目的探究雌激素与星形胶质细胞增殖和侵袭的相关性。方法体外培养星形胶质细胞系U-87,分别进行0、10-12、10-10、10-8和10-6mol/L雌激素处理,通过qPCR、Western blot、ELISA、CCK8法以及Transwell小室法分析相关基因、蛋白质表达以及细胞增殖和侵袭功能。结果相比对照组,雌激素呈浓度依赖性降低细胞因子IL-1β、TNF-α、IL-6的表达,升高NGF、PGE2表达(P<0. 05),抑制水通道蛋白2(AQP2)的表达(P<0. 05);同时相比对照组,雌激素呈浓度依赖性促进星形胶质细胞的增殖和侵袭(P<0. 05),细胞活性显著提高。结论雌激素下调AQP2的表达,下调细胞因子的表达,升高NGF、PGE2表达,促进星形胶质细胞的增殖和侵袭。

Objective To explore the relationships between the estrogen and astrocyte cell proliferation as well as invasion. Methods Astrocyte cell line U-87 was cultured in vitro and treated with 0,10-12,10-10,10-8 and 10-6 mol/L estrogen. The gene and protein expressions were analyzed by qPCR,Western blot,ELISA;and the cell proliferation and invasion was analyzed by CCK-8 kits and transwell experiments. Results Estrogen significantly inhibited IL-1,TNF-α,IL-6 expression and promoted the expression of NGF and PGE2( P<0. 05). Furthermore,estrogen inhibited aquaporin 2( AQP2) expression( P<0. 05). The proliferation and invasion experiments showed that estrogen promoted the U-87 cell proliferation and invasion( P<0. 05). Conclusions Estrogen down-regulates the expression of inflammatory factors,inhibites the expression of AQP2,promotes the proliferation and invasion of U-87 cells.