丝素基双层敷料的制备及其性能

Preparation and properties of silk fibroin based bilayer dressing materials

针对丝素蛋白(SF)吸水性差、力学性能不足等问题,以丝素蛋白为基材,亲水性葡萄糖(Glu)和塑化剂甘油(Gly)为辅料,通过冷冻干燥法制备得到SF/Glu海绵,然后通过蒸发溶剂法制备聚氨酯(PU)薄膜。通过医用热熔胶黏合SF/Glu海绵与PU薄膜获得丝素基双层敷料,并对双层敷料的结构和性能进行分析。结果表明:在Gly质量分数为0.5%,PU质量分数为8%,Glu质量分数为10%条件下制备的双层敷料,其吸水率高达自身质量的12.5倍,溶失率低于2%,保水时间延长至11 h;Glu和SF的相容性较好,Glu可使SF维持较稳定的β-折叠结构;制备的双层敷料无细胞毒性,且具有阻菌功能。

In order to solve poor water absorption and insufficient mechanical properties of silk fibroin(SF),the SF/Glu sponge was prepared through lyophilization with silk fibroin as the substrate and hydrophilic glucose(Glu)and plasticizer glycerol(Gly)as the auxiliary materials.In the meantime,polyurethane(PU)was manufactured into membrane by using the evaporation solvent method.A medical hot melt adhesive was then employed to glue the SF/Glu sponge and PU membrane together to create the bilayer dressing materials,whose structure and properties were analyzed afterwards.The results show that when the mass fraction of Gly is set to 0.5%,that of PU to 8%,and that of Glu to 10%,the water absorption by silk fibroin bilayer dressing material became 12.5 times higher than its own mass.In addition,the bilayer dressing demonstrates a low dissolubility of less than 2%and the water retention time reaches 11 h.Structural characterization reveals that the glucose and silk fibroin have good compatibility and glucose enables silk fibroin to maintain a stableβ-sheet structure.The bilayer dressing bio-safety experiments further indicate that the new dressing material is not cytotoxic and is bacterial-resistant.