基于高通量测序技术的两种水螅水母现场食物研究

Dietary analysis of two hydromedusa in situ based on high-throughput sequencing technique

水母类在自然海区的食物组成是摄食生态学研究的核心问题,本研究利用高通量测序技术分析了管花萨氏水母(Sarsia tubulosa)和八斑芮氏水母(Rathkea octopunctata)的现场食物组成。结果表明:管花萨氏水母和八斑芮氏水母现场摄食食物具有较高的多样性,管花萨氏水母摄食食物31种,八斑芮氏水母29种;两种水母从食物组成上看,属于杂食性生物,其食物包括浮游动物、真菌、浮游植物、陆地植物碎屑等;管花萨氏水母和八斑芮氏水母二者互为捕食对象;二者摄食的食物类群具有相似性,主要来源于节肢动物门和颚足纲,均以太平真宽水蚤为摄食优势种,分别贡献两者食物总量的45.14%和42.42%。高通量测序技术是深入探究水母现场食物组成新的技术手段,两种水母的现场食物组成结果为水母摄食生态学的研究提供了重要参考。

The diet composition of jellyfish is a key issue of feeding ecology research.This paper used high-throughput DNA sequencing technique to identify the food composition of Sarsia tubulosa and Rathkea octopunctata,in situ.The results showed that the food types of Sarsia tubulosa and Rathkea octopunctata were multitudinous.The Sarsia tubulosa predated 31 different species,while the Rathkea octopunctata preyed 29.In term of food composition,the two species of jellyfish were omnivorous organisms,included zooplankton,fungi,phytoplankton,terrestrial plant detritus and so on.The Sarsia tubulosa and Rathkea octopunctata were mutual predators.The food groups of 2 species were similar.Arthropods and Maxillopoda were the most frequently observed phylum and class in the food of both Sarsia tubulosa and Rathkea octopunctata.The dominant species was Eurytemora pacifica,which accounted for 45.14%and 42.42%of the food source,respectively.The utilization of high-throughput sequencing technique is a new and useful tool for studying the diet of jellyfish in situ,and the food composition resulted from 2 jellyfish provides a significant reference for jellyfish feeding ecology.