circ0000231在非小细胞肺癌中的表达及功能研究

Expression and function of circular RNA0000231 in non-small-cell lung cancer

目的:探讨环状RNA_0000231(circ_0000231)在非小细胞肺癌(NSCLC)中的表达及功能。方法:应用RT-qPCR检测circ_0000231在NSCLC组织和细胞系中的表达,将circ_0000231的小干扰RNA(si-circ_0000231)和阴性对照siRNA(NC)分别转染NSCLC细胞,采用CCK-8法、集落形成实验和流式细胞术检测2组细胞的增殖和凋亡情况,并用RT-qPCR和Western blot检测细胞周期蛋白D1(CCND1)和抗凋亡蛋白Bcl-2的表达。结果:分别与癌旁组织和人正常支气管上皮BEAS-2B细胞相比,circ_0000231在NSCLC组织和细胞系中的表达均显著上调(P<0.05)。与NC组相比,si-circ_0000231组的NSCLC细胞活力和集落形成数显著降低,凋亡率显著增加(P<0.05)。此外,RT-qPCR和Western blot检测结果显示转染si-circ_0000231能够抑制NSCLC细胞CCND1和Bcl-2的表达(P<0.01)。结论:circ_0000231在NSCLC组织和细胞中的表达显著升高,敲减circ_0000231的表达能显著抑制NSCLC细胞的增殖。

AIM:To investigate the expression and function of circular RNA_0000231(circ_0000231)in non-small-cell lung cancer(NSCLC).METHODS:RT-qPCR was used to detect the expression of circ_0000231 in the NSCLC tissues and cell lines.circ_0000231 small interfering RNA(si-circ_0000231)or negative control siRNA of circ_0000231(NC)was transfected into the NSCLC cells.The proliferation and apoptosis of the NSCLC cells were detected by CCK-8 assay,colony formation assay and flow cytometry,respectively.The expression of cyclin D1(CCND1)and anti-apoptotic protein Bcl-2 were determined by RT-qPCR and Western blot.RESULTS:The expression of circ_0000231 in the NSCLC tissues and cell lines was significantly up-regulated compared with precancerous tissues and lung epithelial cells BEAS-2B(P<0.05).After transfection of NSCLC cells with si-circ_0000231,the cell viability,colony formation numbers were significantly decreased,and the apoptotic rate in si-circ_0000231 group was significantly increased as compared with NC group(P<0.01).In addition,the results of RT-qPCR and Western blot showed that transfection of si-circ_0000231 inhibited the expression of CCND1 and Bcl-2(P<0.01).CONCLUSION:The expression of circ_0000231 is significantly increased in the NSCLC tissues and cells.Knock-down of circ_0000231 expression significantly inhibits the proliferation of NSCLC cells.