基于体外模拟肠道微生态体系比较不同果蔬全粉的益生元功效

The Comparison of Prebiotic Roles of Different Vegetable and Fruit Powders Using in Vitro Simulation Intestinal Microecology System

体外模拟肠道系统逐渐成为研究肠道菌群及其代谢产物的一种操作简便、重现性好的方法。本研究通过建立体外模拟肠道微生态体系,对丝瓜、桑葚、枸杞和香菇冻干粉的益生元功效展开研究。结果表明,经24 h体外发酵后,丝瓜冻干粉对乳酸杆菌属、普氏菌属、拟杆菌属数量和丙酸产量增加的促进作用显著高于其它果蔬组,桑葚对双歧杆菌数量和乙酸产量增加的促进作用显著高于其它果蔬组。以上结果说明基于体外模拟肠道微生态体系,丝瓜和桑葚冻干全粉均具有较为显著的益生元功效。

The in vitro simulation intestinal microecology system is available method of easy-opeartion and good-reproducibility, which can be used to study intestinal microbiota and its metabolites gradually. In this study, the beneficial prebiotic effects of Luffa cylindrica, Morus alba, Lycium barbarum and Lentinus edodes freeze-dried powders were studied using a system of in vitrosimulation intestinal microecology. The results showed that Luffa cylindrica significantly increased the more numbers of Lactobacillus, Prevotella, Bacteroides and higherpropionate acidcontent with in vitro fermentationfor 24 hours, which was better than other vegetable and fruit powders did. Meanwhile, Morus alba promoted a largerincrease(P<0.05) of the number of Bifidobacterium and the production of acetate acid than others did. These findings suggest that Luffa cylindrica and Morus alba probably display betterprebiotic effects on intestinal microbiotaon the basis of in vitro simulation intestinal microecology system.