黑色小扁豆中不同结合态酚类含量、花青素组成及其抗氧化活性研究

Studies on the Contents of Different Binding Phenolics, Anthocyanin Compose of Black Lentil and Their Antioxidant Activity

黑色小扁豆因含有丰富的营养素、微量营养素和植物化学物成分而受到研究者的关注。为深入了解小扁豆的营养价值,对小扁豆中不同结合形态的酚类化合物含量及其抗氧化活性(亚铁离子还原能力、DPPH自由基清除能力、ABTS自由基清除能力)进行测定。结果表明,黑色小扁豆中不同存在形式的酚类提取物含量差异显著。酯键合态酚类提取物中含有较高的酚酸含量(1.61 mg/g),碱水解结合态酚类提取物具有较高的黄酮含量(1.07 mg/g)及抗氧化活性【亚铁离子还原能力(43.80 mmol/g)、DPPH自由基清除能力(2.13 mg/g)、ABTS清除能力(3.92 mg/g)】。对黑色小扁豆中存在的花青素进行提取和分析鉴定,其含有的花青素主要为飞燕草-3-O-(2-O-β-D-吡喃葡萄糖基-α-L-吡喃阿拉伯糖苷)和矢车菊素衍生物。

Lentil(Lens culinaris) is widely consumed due to its excellent sources of macronutrient, micronutrients and phytochemicals. In order to better understand its nutritional value, the present study investigated the free, conjugated and bound phenolic content of the black lentil and their antioxidant activity(ferric reducing antioxidant power(FRAP),DPPH(1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity, ABTS[2, 2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity]. Ester conjugated phenolic showed highest levels of phenolic contents(1.61 mg/g), while base hydrolyzed bound phenolic presented the highest levels of flavonoid contents(1.07 mg/g) and antioxidant activities[FRAP(43.80 mmol/g), DPPH(2.13 mg/g), ABTS(3.92 mg/g)]. In addition, two major anthocyanins were identified as delphinidin 3-O-(2-O-β-D-glucopyranosyl-α-L-arabinopyranoside) and cyanidin derivatives in the acidified methanolic extract of black lentil.