响应曲面法对玉米秸秆稀酸水解还原糖条件的优化

Optimization of Corn Straw Cellulose Dilute Acid Hydrolytic into Reducing Sugar by Response Surface Methodology

目的研究玉米秸秆纤维素在稀酸水解过程中各个因素对还原糖产率的影响,并对玉米秸秆纤维素稀酸水解条件进行优化.方法针对我国辽宁沈阳地区的农作物种植与能源需求现状,利用响应曲面设计试验方法(RSM),选择酸投加量、搅拌温度、水解时间作为试验因子,建立3因素3水平响应曲面回归模型进行分析,将还原糖产率作为响应值并进行曲面分析.结果3个影响因子对还原糖产率均具有显著影响(P<0.05).所得回归方程R2值为0.9021,P<0.05,足够精度值为5.962,说明模型高度显著,可以在设计范围内对响应值进行预测.模型预测最佳水解条件为温度58.21℃,水解时间2.86 h,稀酸投加量112.55mL.利用最佳水解条件进行验证试验,溶液总体积为165 mL,经过试验验证:玉米秸秆稀酸水解的还原糖产率为60.45%,此时,水解液中还原糖质量浓度为0.015 g/mL.结论搅拌温度与水解时间两因素对还原糖产率影响较为明显,该模型可以较好地预测出玉米秸秆纤维素稀酸水解的最佳反应条件与还原糖产率.

The effects of various factors on the yield of reducing sugar in the process of dilute acid hydrolysis of corn stover cellulose were studied,and the hydrolysis conditions of stalk cellulose were optimized.In response to the current situation of crop planting and energy demand in Shenyang,Liaoning province,the response surface method(RSM)was utilized to design experiment.Acid dosage,stirring temperature and hydrolysis time were selected as experimental factors.A 3-factor 3 level response surface regression model was established and reducing sugar yield was used as response value.3 impact factors had a significant effect on the yield of reducing sugar(P<0.05),the regression equation R 2 value was 0.9021,P<0.05,and the sufficient precision value was 5.962,indicating that the response value can be predicted within the design range by model.The optimum hydrolysis conditions for the model were as follows:temperatures 58.21℃,hydrolysis time 2.86 h,dilute acid dosage 112.55 mL.The best hydrolysis conditions were used to design the verification experiment.In the verification experiment,the total volume of the solution was 165 mL,the yield of reducing sugar is 60.45%,and the concentration of reducing sugar in the hydrolysate is 0.015g/mL.Stirring temperature and hydrolysis time have a clear influence on the yield of reducing sugar.This model can predict the optimal reaction conditions and the reducing sugar yield for corn stover dilute acid hydrolysis of cellulose.