ICR小鼠自发运动、探索行为和焦虑情绪的检测及其参考值的确立

Detection of Spontaneous Movement,Exploration Behavior and Anxiety in ICR Mice and Establishment of Reference Range of Corresponding Indicators

神经行为学测试极易受到试验中各类因素的影响,阻碍研究人员间的相互参考、比较。因此,研究旨在观察正常状态下ICR小鼠的自发运动、探索行为和焦虑情绪,为今后的相关研究奠定基础。利用TM-Vision行为学视频分析系统对90只ICR小鼠进行旷场试验,记录ICR小鼠5 min内的活动情况,建立各指标的检测值及95%置信区间,并分析其两性差异。高架十字迷宫测试方法同上。研究条件控制严格,行为学测量结果稳定、科学,具备较好的重复性和可信度,可以提供有价值的参考,且ICR小鼠在自发运动、探索行为和焦虑情绪上不存在两性差异。

Neurobehavioral testing was highly susceptible to various factors in the trial,hindering mutual reference and comparison among researchers.Therefore,to observe the spontaneous movement,exploration behavior and anxiety of ICR mice under normal conditions,and lay a foundation for future research and provide valuable reference,90 ICR mice were subjected to open field test using TM-Vision behavioral video analysis system,and the activity of ICR mice within 5 min was recorded.The detection values and 95%confidence intervals of each index were established,and the gender differences were analyzed.The plus maze test method was the same as above.Under the conditions of our experiment,the behavioral measurement results were stable and scientific,with good reprodu-cibility and credibility,and there were no gender differences in spontaneous movement,exploration behavior and anxiety in ICR mice.