淄博地区血小板捐献汉族人群HLA和HPA系统基因多态性的研究

Genetic polymorphism of the HLA-A,B and HPA1～17 in platelet donors of Zibo Han population

目的了解淄博地区血小板捐献者HLA-A、HLA-B和HPA1~17系统基因多态性分布特点,初步建立淄博地区血小板捐献者已知HLA、HPA基因型资料库,进一步扩充山东省区域血小板捐献者资料库容量。方法分别采用PCR-SBT和PCR-SSP方法对200名无血缘关系的单采血小板捐献者进行HLA-A、HLA-B和HPA1~17系统基因分型,计算基因频率、基因型频率。结果共检出HLA-A位点低分辨等位基因15个,HLA-B位点低分辨等位基因29个,其中HLA-A位点基因频率居前五位的是A~*02、A~*24、A~*11、A~*30、A~*33,基因频率分别为0.2600、0.1725、0.1375、0.1350、0.0700;HLA-B位点基因频率居前五位的是B~*13、B~*15、B~*40、B~*44、B~*51,基因频率分别为0.1625、0.1325、0.1300、0.0675、0.0625。HPA1~17中,HPA4a、HPA7a^14a、HPA16a、HPA17a呈单特异性,未检测出相应的对偶基因抗原HPA-b;在HPA1~17HPA1~17中,杂合度最高的是HPA15,基因型HPA15a/15a、HPA15a/15b、HPA15b/15b频率分别是0.275、0.530、0.195;其次为HPA3,基因型HPA3a/3a、HPA3a/3b、HPA3b/3b频率分别是0.345、0.500、0.155。结论分析探知了淄博地区血小板捐献者HLA-A、HLA-B和HPA1~17的分布特点,为免疫性血小板输注无效患者提供匹配的血小板和建立山东省区域性血小板捐献者资料库提供帮助。

Objective To understand the genetic polymorphism of HLA-A,HLA-B alleles and HPA1~17 in Zibo area and to establish a platelet donor data bank of Zibo area with HLA and HPA typing,further expanding the capacity of platelet donor bank in Shandong province.Methods The technique of polymerase chain reaction sequencing based typing(PCR-SBT)and PCR-sequence specific primers(PCR-SSP)methods were respectively adopted in genotyping HLA-A,HLA-B and HPA1~17.Samples from 200 unrelated platelet donors were collected from Zibo region.The gene and genotypic frequencies were calculated.Results The numbers of low-resolution alleles identified were 15 for HLA-A,29 for HLA-B,respectively.The top five gene frequencies were A~*02、A~*24、A~*11、A~*30、A~*33,with a gene frequency in 0.2600、0.1725、0.1375、0.1350、0.0700,respectively.For the HLA-B locus,The top five gene frequencies were B~*13、B~*15、B~*40、B~*44、B~*51,with a gene frequency of 0.1625、0.1325、0.1300、0.0675、0.0625,respectively.Monomorphic HPA4 a、7 a-14 a、16 a、17 a were found in the samples,but none of corresponding allele antigen HPA-b was detected.HPA-15 exhibited the greatest heterozygosity with a genotype frequency of 0.275、0.530 and 0.195 for HPA15 a/15 a,HPA15 a/15 b,and HPA15 b/15 b,respectively.HPA-3 showed the second greatest heterozygosity with a genotype frequency of 0.345、0.500 and 0.155 for HPA3 a/3 a,HPA3 a/3 b and HPA3 b/3 b,respectively.Conclusion The distribution characteristics of HLA-A,HLA-B and HPA1~17 in platelet donors in Zibo area were analyzed and explored,for providing matching platelets for Invalid immune platelet transfusion patients,and providing help for the establishment of a regional platelet donor bank in Shandong Province.