凋亡因子FADD蛋白与Caspase-7蛋白在胃腺癌中的表达及意义

EXPRESSION AND SIGNIFICANCE OF APOPTOSIS FACTOR FADD PROTEIN AND CASPASE-7 PROTEIN IN GASTRIC ADENOCARCINOMA

目的:探讨胃腺癌中Fas相关死亡结构域蛋白(FADD)与半胱氨酸蛋白酶-7蛋白(Caspase-7)的表达及意义。方法:采用免疫组化S P法检测60例胃腺癌组织及50例正常胃黏膜组织F A D D蛋白与C a s p a s e-7蛋白的表达情况,分析二者与胃腺癌临床病理特征的关系,以及胃腺癌组织中二者表达的相关性。结果:FADD蛋白和Caspase-7蛋白阳性产物均为棕黄色颗粒状,主要位于细胞质。胃腺癌组织FADD蛋白和Caspase-7蛋白的阳性表达率均明显低于正常胃黏膜组织(38.3%vs 60.0%,46.7%vs 68.0%,P<0.05)。FADD蛋白、Caspase-7蛋白表达与胃腺癌患者性别、年龄无关(P>0.05),与胃腺癌分化程度、T N M分期、淋巴结转移、浸润深度有关。Spearman相关分析结果显示,胃腺癌FADD蛋白和Caspase-7蛋白表达呈正相关关系(r=0.362,P<0.05)。结论:FADD蛋白与Caspase-7蛋白低表达可能在胃腺癌的发生发展过程中具有协同作用。

Objective:To investigate the expression and significance of Fas-associated death domain(FADD)protein and Caspase-7 protein in gastric adenocarcinoma Methods:The expression of FADD protein and Caspase-7 protein in 60 cases of gastric adenocarcinoma and 50 cases of normal gastric mucosa were detected by immunohistochemical SP method.The relationships between FADD protein,Caspase-7 protein and clinicopathological features of gastric adenocarcinoma,as well as the correlations between FADD protein and Caspase-7 protein in gastric adenocarcinoman were analyzed.Results:The positive products of FADD protein and Caspase-7 protein were all brownish yellow granules,mainly located in the cytoplasm.The positive expression rate of FADD protein and Caspase-7 protein in gastric adenocarcinoma were significantly lower than that in normal gastric mucosa tissues(38.3%vs 60.0%,46.7%vs 68.0%,P<0.05).In gastric adenocarcinoma,the FADD protein and Caspase-7 protein expression were not correlated with age and gender(P>0.05);but related to the differentiation,TNM stage,lymph node metastasis and infiltration depth(P<0.05).Spearman correlation analysis showed that there had positive correlation between expression of FADD protein and caspase-7 protein in gastric adenocarcinoma(r=0.362,P<0.05).Conclusions:Low expression of FADD protein and Caspase-7 protein may play a synergistic role in the development of gastric adenocarcinoma.